Article,

Practical Problem For Assessing Antioxidant Enzymes

, and .
Weekly Science, (September 2013)

Abstract

Monitoring of total antioxidant capacity in biological fluid is a popular method of modern medicine for assessing oxidative stressThe estimation requires proper infrastructure for maintaining samples prior to assay. Lack of proper infrastructure for conducting epidemiologic studies in relation to maintenance of biological samples at study site is common in third world countries. Although antioxidant markers like superoxide dismutase and glutathione peroxidase are reported to be stable at -80°C, we speculated its deterioration upon storage at 2- 8°C. The aim of the present study was to determine whether antioxidant markers retain its activity upon storage at 2- 8°C. Blood was collected from 26 healthy subjects (age 25-45 years).

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