%0 Journal Article %1 bheeram2019cytotoxic %A Bheeram, Vema Reddy %A Malla, Rama Rao %A Seema, Kumari %A Abhijit, Saha %A Mukkamala, Saratchandra Babu %D 2019 %J Innovation and Research in Biomedical Engineering (IRBM) %K myown %P 270-278 %R 10.1016/j.irbm.2019.05.001 %T Cytotoxic Effect of Photoluminescent RE3+ Doped Ca3(PO4)2 Nanorods on Breast Cancer Cell Lines %U https://www.sciencedirect.com/science/article/abs/pii/S1959031818303543 %V 40 %X Background Breast cancer reported in the young women exhibits high local and distant recurrence and a poor prognosis. Rare earth doped calcium phosphate phosphors have been extensively investigated due to their unique applications in biomedicine. Methods In the current study, Tb3+, Ce3+ doped Ca3(PO4)2 phosphor were prepared by hydrothermal method at 150 °C using citric acid as additive and characterized by PXRD, FT-IR, TG-DTA, EDX, TEM and PL techniques. The photoluminescence properties of Tb3+, Ce3+ doped Ca3(PO4)2 phosphor was investigated upon photo excitation at 240 nm. Antiproliferative activity was evaluated by MTT, BrdU proliferation, ELISA, Methylene blue and caspase-3 assays. Results Ca3(PO4)2:Tb3+, Ce3+ phosphor exhibited needle like morphology with length and width ∼100-500 nm and ∼40-50 nm, respectively. It exhibited green emission at 550 nm corresponding to 5D4→7F5 transition with the CIE coordinates (x, y) as (0.284, 0.614). It also showed remarkable concentration dependent cytotoxicity against MCF-7 as well as MDA-MB 231 cells with negligible cytotoxicity compared to MCF-12A, a human epithelial healthy cell line. It reduced the proliferative index of both cell lines in a concentration dependent manner by inhibiting DNA synthesis and Ki67 protein. It also induced distinct apoptotic changes in the morphology of cell and nucleus and also activated the caspase-3 activity in breast cancer cell lines. Conclusion The results suggest that Ca3(PO4)2:Tb3+, Ce3+ phosphor may be useful for therapeutic application in clinical settings.

@article{bheeram2019cytotoxic, abstract = {Background Breast cancer reported in the young women exhibits high local and distant recurrence and a poor prognosis. Rare earth doped calcium phosphate phosphors have been extensively investigated due to their unique applications in biomedicine. Methods In the current study, Tb3+, Ce3+ doped Ca3(PO4)2 phosphor were prepared by hydrothermal method at 150 °C using citric acid as additive and characterized by PXRD, FT-IR, TG-DTA, EDX, TEM and PL techniques. The photoluminescence properties of Tb3+, Ce3+ doped Ca3(PO4)2 phosphor was investigated upon photo excitation at 240 nm. Antiproliferative activity was evaluated by MTT, BrdU proliferation, ELISA, Methylene blue and caspase-3 assays. Results Ca3(PO4)2:Tb3+, Ce3+ phosphor exhibited needle like morphology with length and width ∼100-500 nm and ∼40-50 nm, respectively. It exhibited green emission at 550 nm corresponding to 5D4→7F5 transition with the CIE coordinates (x, y) as (0.284, 0.614). It also showed remarkable concentration dependent cytotoxicity against MCF-7 as well as MDA-MB 231 cells with negligible cytotoxicity compared to MCF-12A, a human epithelial healthy cell line. It reduced the proliferative index of both cell lines in a concentration dependent manner by inhibiting DNA synthesis and Ki67 protein. It also induced distinct apoptotic changes in the morphology of cell and nucleus and also activated the caspase-3 activity in breast cancer cell lines. Conclusion The results suggest that Ca3(PO4)2:Tb3+, Ce3+ phosphor may be useful for therapeutic application in clinical settings.}, added-at = {2021-09-26T05:01:46.000+0200}, author = {Bheeram, Vema Reddy and Malla, Rama Rao and Seema, Kumari and Abhijit, Saha and Mukkamala, Saratchandra Babu}, biburl = {https://www.bibsonomy.org/bibtex/23ff03d04442dfeefcbef69eb6c54d512/vemareddy-b1908}, doi = {10.1016/j.irbm.2019.05.001}, interhash = {9511e5b937d5e18e7b97c3e16c1b1055}, intrahash = {3ff03d04442dfeefcbef69eb6c54d512}, issn = {1959-0318}, journal = {Innovation and Research in Biomedical Engineering (IRBM)}, keywords = {myown}, language = {English}, month = {October}, pages = {270-278}, timestamp = {2021-09-26T05:01:46.000+0200}, title = {Cytotoxic Effect of Photoluminescent RE3+ Doped Ca3(PO4)2 Nanorods on Breast Cancer Cell Lines}, url = {https://www.sciencedirect.com/science/article/abs/pii/S1959031818303543}, volume = 40, year = 2019 }