Recombinant nuclear polyhedrosis viruses (NPVs) expressing insect-selective toxins, hormones, or enzymes could enhance their insecticidal properties. We have constructed a recombinant, polyhedrin-positive Autographa californica NPV (AcNPV) that is orally infectious and expresses an insect-selective toxin (AaIT), isolated from the scorpion Androctonus australis, under the control of the p10 promoter. Bioassays with the recombinant baculovirus on 2nd instar larvae of Heliothis virescens demonstrated a significant decrease in the time to kill (LT50 88.0 hours) compared to wild-type AcNPV (LT50 125 hours). Production of AaIT was confirmed by western blot analysis of larval hemolymph from infected H. virescens, and bioassays with larvae of Sarcophaga falculata.
%0 Journal Article
%1 McCutchen1991
%A McCutchen, B. F.
%A Choudary, P. V.
%A Crenshaw, R.
%A Maddox, D.
%A Kamita, S. G.
%A Palekar, N.
%A Volrath, S.
%A Fowler, E.
%A Hammock, B. D.
%A Maeda, S.
%D 1991
%J Biotechnology (N Y)
%K Animals; Baculoviridae, genetics; Base Sequence; Cell Line; Immunoblotting; Larva, drug effects; Lepidoptera, Molecular Sequence Data; Neurotoxins; Oligonucleotide Probes; Pest Control, methods; Recombinant Proteins, isolation /&/ purification/pharmacology; Recombination, Genetic; Restriction Mapping; Scorpion Venoms, genetics/isolation Transfection
%N 9
%P 848--852
%T Development of a recombinant baculovirus expressing an insect-selective neurotoxin: potential for pest control.
%V 9
%X Recombinant nuclear polyhedrosis viruses (NPVs) expressing insect-selective toxins, hormones, or enzymes could enhance their insecticidal properties. We have constructed a recombinant, polyhedrin-positive Autographa californica NPV (AcNPV) that is orally infectious and expresses an insect-selective toxin (AaIT), isolated from the scorpion Androctonus australis, under the control of the p10 promoter. Bioassays with the recombinant baculovirus on 2nd instar larvae of Heliothis virescens demonstrated a significant decrease in the time to kill (LT50 88.0 hours) compared to wild-type AcNPV (LT50 125 hours). Production of AaIT was confirmed by western blot analysis of larval hemolymph from infected H. virescens, and bioassays with larvae of Sarcophaga falculata.
@article{McCutchen1991,
abstract = {Recombinant nuclear polyhedrosis viruses (NPVs) expressing insect-selective toxins, hormones, or enzymes could enhance their insecticidal properties. We have constructed a recombinant, polyhedrin-positive Autographa californica NPV (AcNPV) that is orally infectious and expresses an insect-selective toxin (AaIT), isolated from the scorpion Androctonus australis, under the control of the p10 promoter. Bioassays with the recombinant baculovirus on 2nd instar larvae of Heliothis virescens demonstrated a significant decrease in the time to kill (LT50 88.0 hours) compared to wild-type AcNPV (LT50 125 hours). Production of AaIT was confirmed by western blot analysis of larval hemolymph from infected H. virescens, and bioassays with larvae of Sarcophaga falculata.},
added-at = {2014-07-19T20:46:36.000+0200},
author = {McCutchen, B. F. and Choudary, P. V. and Crenshaw, R. and Maddox, D. and Kamita, S. G. and Palekar, N. and Volrath, S. and Fowler, E. and Hammock, B. D. and Maeda, S.},
biburl = {https://www.bibsonomy.org/bibtex/2b0c249d4b0498e659e387c95e7bb6ba4/ar0berts},
groups = {public},
institution = {Department of Entomology, University of California, Davis 95616.},
interhash = {9991754fa969730d166fb31d0838e183},
intrahash = {b0c249d4b0498e659e387c95e7bb6ba4},
journal = {Biotechnology (N Y)},
keywords = {Animals; Baculoviridae, genetics; Base Sequence; Cell Line; Immunoblotting; Larva, drug effects; Lepidoptera, Molecular Sequence Data; Neurotoxins; Oligonucleotide Probes; Pest Control, methods; Recombinant Proteins, isolation /&/ purification/pharmacology; Recombination, Genetic; Restriction Mapping; Scorpion Venoms, genetics/isolation Transfection},
language = {eng},
medline-pst = {ppublish},
month = Sep,
number = 9,
pages = {848--852},
pmid = {1367359},
timestamp = {2014-07-19T20:46:36.000+0200},
title = {Development of a recombinant baculovirus expressing an insect-selective neurotoxin: potential for pest control.},
username = {ar0berts},
volume = 9,
year = 1991
}