%0 Journal Article %1 ISI:000256211000001 %A Altincicek, Boran %A Vilcinskas, Andreas %D 2008 %J FRONTIERS IN ZOOLOGY %K imported %T Comparative analysis of septic injury-inducible genes in phylogenetically distant model organisms of regeneration and stem cell research, the planarian Schmidtea mediterranea and the cnidarian Hydra vulgaris %V 5 %X Background: The planarian Schmidtea mediterranea and the cnidarian Hydra vulgaris have emerged as valuable model organisms in regeneration and stem cell research because of their prominent ability to regenerate a complete organism from any small body fragment. Under natural conditions wounding may result from predator attacks. These injuries open their innermost to a wide array of microbes present in the environment. Therefore, we established the hypothesis that regeneration processes may be linked to or at least accompanied by innate immune responses. In order to screen for septic wounding inducible genes we dissected individuals using a scalpel in the presence of a crude bacterial lipopolysaccharide preparation that is commonly used to elicit innate immune responses in animals and applied the suppression subtractive hybridization technique that selectively amplifies cDNAs of differentially expressed genes. Results: This analysis revealed the induced expression of 27 genes in immune c

@article{ISI:000256211000001, abstract = {Background: The planarian Schmidtea mediterranea and the cnidarian Hydra vulgaris have emerged as valuable model organisms in regeneration and stem cell research because of their prominent ability to regenerate a complete organism from any small body fragment. Under natural conditions wounding may result from predator attacks. These injuries open their innermost to a wide array of microbes present in the environment. Therefore, we established the hypothesis that regeneration processes may be linked to or at least accompanied by innate immune responses. In order to screen for septic wounding inducible genes we dissected individuals using a scalpel in the presence of a crude bacterial lipopolysaccharide preparation that is commonly used to elicit innate immune responses in animals and applied the suppression subtractive hybridization technique that selectively amplifies cDNAs of differentially expressed genes. Results: This analysis revealed the induced expression of 27 genes in immune c}, added-at = {2008-10-20T12:44:34.000+0200}, author = {Altincicek, Boran and Vilcinskas, Andreas}, biburl = {https://www.bibsonomy.org/bibtex/237f54209d8ebd72d4b0fdc1b2b3b5ddf/ifzappliedentomol}, interhash = {999d02e7311787891f0d06f56dfaf990}, intrahash = {37f54209d8ebd72d4b0fdc1b2b3b5ddf}, issn = {1742-9994}, journal = {FRONTIERS IN ZOOLOGY}, keywords = {imported}, timestamp = {2008-10-20T12:44:34.000+0200}, title = {Comparative analysis of septic injury-inducible genes in phylogenetically distant model organisms of regeneration and stem cell research, the planarian Schmidtea mediterranea and the cnidarian Hydra vulgaris}, volume = 5, year = 2008 }