%0 Journal Article %1 Khanna2006a %A Khanna, S. %A Srivastava, A. K. %D 2006 %J Biochem. Eng. J. %K imported %N 3 %P 197--203 %T Computer simulated fed-batch cultivation for over production of PHB: A comparison of simultaneous and alternate feeding of carbon and nitrogen %V 27 %X Batch kinetics for cultivation of Ralstonia eutropha, NRRL B 14690 featured 19.7 g/l biomass and 10.89 g/l PHB accumulation in 60 h under statistically optimized culture conditions. For in-depth understanding of culture dynamics and for suitable reactor design a mathematical model was developed. The model was highly instrumental in guiding the appropriate experiments to optimize the process with minimum efforts. Offline computer simulated fed-batch cultivations allowed selection of two different nutrient (nitrogen (as urea) and fructose) feeding strategies. Fed-batch fermentation, conducted by feeding nitrogen (as urea) (8 g/l) at the rate of 70 ml/h and fructose (300 g/l) at the rate of 80 ml/h simultaneously yielded 17.57 g/l PHB in 50 h with an overall productivity of 0.35 g/l h. In another strategy, alternate feeding of the nitrogen (as urea) (15 g/l) and fructose (350 g/l) at a flow rate of 80 ml/h resulted in PUB accumulation of 18.46 g/l in 40 h resulting in overall productivity of 0.46 g/l h. Model-based fed-batch operation was very helpful for over producing PHB by R. eutropha. The increase in PHB productivity in fed-batch cultivation was almost 2.6 times as compared to batch. (c) 2005 Elsevier B.V. All rights reserved.

@article{Khanna2006a, abstract = {Batch kinetics for cultivation of Ralstonia eutropha, NRRL B 14690 featured 19.7 g/l biomass and 10.89 g/l PHB accumulation in 60 h under statistically optimized culture conditions. For in-depth understanding of culture dynamics and for suitable reactor design a mathematical model was developed. The model was highly instrumental in guiding the appropriate experiments to optimize the process with minimum efforts. Offline computer simulated fed-batch cultivations allowed selection of two different nutrient (nitrogen (as urea) and fructose) feeding strategies. Fed-batch fermentation, conducted by feeding nitrogen (as urea) (8 g/l) at the rate of 70 ml/h and fructose (300 g/l) at the rate of 80 ml/h simultaneously yielded 17.57 g/l PHB in 50 h with an overall productivity of 0.35 g/l h. In another strategy, alternate feeding of the nitrogen (as urea) (15 g/l) and fructose (350 g/l) at a flow rate of 80 ml/h resulted in PUB accumulation of 18.46 g/l in 40 h resulting in overall productivity of 0.46 g/l h. Model-based fed-batch operation was very helpful for over producing PHB by R. eutropha. The increase in PHB productivity in fed-batch cultivation was almost 2.6 times as compared to batch. (c) 2005 Elsevier B.V. All rights reserved. }, added-at = {2010-12-02T09:30:05.000+0100}, author = {Khanna, S. and Srivastava, A. K.}, biburl = {https://www.bibsonomy.org/bibtex/29db14ded165939668d58ce61df68a277/afranz}, interhash = {aa53b2b1fecdc33eb1247c75ec7fb45d}, intrahash = {9db14ded165939668d58ce61df68a277}, journal = {Biochem. Eng. J.}, keywords = {imported}, number = 3, pages = {197--203}, timestamp = {2010-12-02T09:30:06.000+0100}, title = {Computer simulated fed-batch cultivation for over production of PHB: A comparison of simultaneous and alternate feeding of carbon and nitrogen}, volume = 27, year = 2006 }