%0 Journal Article %1 WOS:000463883500028 %A Utino, Fabiane Leonel %A Garcia, Marina %A Velho, Paulo Eduardo Neves Ferreira %A da Costa Franca, Andrea Fernandes Eloy %A Stelini, Rafael Fantelli %A Pelegati, Vitor Bianchin %A Cesar, Carlos Lenz %A de Souza, Elemir Macedo %A Cintra, Maria Leticia %A Damiani, Gislaine Vieira %C 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98225 USA %D 2018 %I SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS %J JOURNAL OF BIOMEDICAL OPTICS %K diseases; generation; leprosy} sarcoidosis; second-harmonic tuberculoid {granulomatous %N 12 %R 10.1117/1.JBO.23.12.126001 %T Second-harmonic generation imaging analysis can help distinguish sarcoidosis from tuberculoid leprosy %V 23 %X Sarcoidosis and tuberculoid leprosy (TL) are prototypes of granulomatous inflammation in dermatology, which embody one of the histopathology limitations in distinguishing some diseases. Recent advances in the use of nonlinear optical microscopy in skin have enabled techniques, such as second-harmonic generation (SHG), to become powerful tools to study the physical and biochemical properties of skin. We use SHG images to analyze the collagen network, to distinguish differences between sarcoidosis and TL granulomas. SHG images obtained from skin biopsies of 33 patients with TL and 24 with sarcoidosis retrospectively were analyzed using first-order statistics (FOS) and second-order statistics, such as gray-level co-occurrence matrix (GLCM). Among the four parameters evaluated (optical density, entropy, contrast, and second angular moment), only contrast demonstrated statistical significance, being higher in sarcoidosis (p = 0.02; 4908.31 versus 2822.17). The results may indicate insufficient differentiating power for most tested FOS and GLCM parameters in classifying sarcoidosis and TL granulomas, when used individually. But in combination with histopathology (H&E and complementary stains, such as silver and fast acid stains), SHG analysis, like contrast, can contribute to distinguishing between these diseases. This study can provide a way to evaluate collagen distribution in granulomatous diseases. (c) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.

@article{WOS:000463883500028, abstract = {Sarcoidosis and tuberculoid leprosy (TL) are prototypes of granulomatous inflammation in dermatology, which embody one of the histopathology limitations in distinguishing some diseases. Recent advances in the use of nonlinear optical microscopy in skin have enabled techniques, such as second-harmonic generation (SHG), to become powerful tools to study the physical and biochemical properties of skin. We use SHG images to analyze the collagen network, to distinguish differences between sarcoidosis and TL granulomas. SHG images obtained from skin biopsies of 33 patients with TL and 24 with sarcoidosis retrospectively were analyzed using first-order statistics (FOS) and second-order statistics, such as gray-level co-occurrence matrix (GLCM). Among the four parameters evaluated (optical density, entropy, contrast, and second angular moment), only contrast demonstrated statistical significance, being higher in sarcoidosis (p = 0.02; 4908.31 versus 2822.17). The results may indicate insufficient differentiating power for most tested FOS and GLCM parameters in classifying sarcoidosis and TL granulomas, when used individually. But in combination with histopathology (H&E and complementary stains, such as silver and fast acid stains), SHG analysis, like contrast, can contribute to distinguishing between these diseases. This study can provide a way to evaluate collagen distribution in granulomatous diseases. (c) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.}, added-at = {2022-05-23T20:00:14.000+0200}, address = {1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98225 USA}, author = {Utino, Fabiane Leonel and Garcia, Marina and Velho, Paulo Eduardo Neves Ferreira and da Costa Franca, Andrea Fernandes Eloy and Stelini, Rafael Fantelli and Pelegati, Vitor Bianchin and Cesar, Carlos Lenz and de Souza, Elemir Macedo and Cintra, Maria Leticia and Damiani, Gislaine Vieira}, biburl = {https://www.bibsonomy.org/bibtex/2060b746792c81ff089c476bdd7a8e062/ppgfis_ufc_br}, doi = {10.1117/1.JBO.23.12.126001}, interhash = {aacceb765eb101bf4df4ca095e58e338}, intrahash = {060b746792c81ff089c476bdd7a8e062}, issn = {1083-3668}, journal = {JOURNAL OF BIOMEDICAL OPTICS}, keywords = {diseases; generation; leprosy} sarcoidosis; second-harmonic tuberculoid {granulomatous}, number = 12, publisher = {SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS}, pubstate = {published}, timestamp = {2022-05-23T20:00:14.000+0200}, title = {Second-harmonic generation imaging analysis can help distinguish sarcoidosis from tuberculoid leprosy}, tppubtype = {article}, volume = 23, year = 2018 }