%0 Journal Article %1 Li2011Systematic %A Li, Zhijian %A Vizeacoumar, Franco J. %A Bahr, Sondra %A Li, Jingjing %A Warringer, Jonas %A Vizeacoumar, Frederick S. %A Min, Renqiang %A VanderSluis, Benjamin %A Bellay, Jeremy %A DeVit, Michael %A Fleming, James A. %A Stephens, Andrew %A Haase, Julian %A Lin, Zhen-Yuan %A Baryshnikova, Anastasia %A Lu, Hong %A Yan, Zhun %A Jin, Ke %A Barker, Sarah %A Datti, Alessandro %A Giaever, Guri %A Nislow, Corey %A Bulawa, Chris %A Myers, Chad L. %A Costanzo, Michael %A Gingras, Anne-Claude %A Zhang, Zhaolei %A Blomberg, Anders %A Bloom, Kerry %A Andrews, Brenda %A Boone, Charles %D 2011 %I Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. %J Nat Biotech %K yeast yeast-gene-dup %N 4 %P 361--367 %R 10.1038/nbt.1832 %T Systematic exploration of essential yeast gene function with temperature-sensitive mutants %U http://dx.doi.org/10.1038/nbt.1832 %V 29 %X Conditional temperature-sensitive (ts) mutations are valuable reagents for studying essential genes in the yeast Saccharomyces cerevisiae. We constructed 787 ts strains, covering 497 (∼45\%) of the 1,101 essential yeast genes, with ∼30\% of the genes represented by multiple alleles. All of the alleles are integrated into their native genomic locus in the S288C common reference strain and are linked to a kanMX selectable marker, allowing further genetic manipulation by synthetic genetic array (SGA)-based, high-throughput methods. We show two such manipulations: barcoding of 440 strains, which enables chemical-genetic suppression analysis, and the construction of arrays of strains carrying different fluorescent markers of subcellular structure, which enables quantitative analysis of phenotypes using high-content screening. Quantitative analysis of a GFP-tubulin marker identified roles for cohesin and condensin genes in spindle disassembly. This mutant collection should facilitate a wide range of systematic studies aimed at understanding the functions of essential genes.

@article{Li2011Systematic, abstract = {Conditional temperature-sensitive (ts) mutations are valuable reagents for studying essential genes in the yeast Saccharomyces cerevisiae. We constructed 787 ts strains, covering 497 (∼45\%) of the 1,101 essential yeast genes, with ∼30\% of the genes represented by multiple alleles. All of the alleles are integrated into their native genomic locus in the {S288C} common reference strain and are linked to a {kanMX} selectable marker, allowing further genetic manipulation by synthetic genetic array ({SGA})-based, high-throughput methods. We show two such manipulations: barcoding of 440 strains, which enables chemical-genetic suppression analysis, and the construction of arrays of strains carrying different fluorescent markers of subcellular structure, which enables quantitative analysis of phenotypes using high-content screening. Quantitative analysis of a {GFP}-tubulin marker identified roles for cohesin and condensin genes in spindle disassembly. This mutant collection should facilitate a wide range of systematic studies aimed at understanding the functions of essential genes.}, added-at = {2018-12-02T16:09:07.000+0100}, author = {Li, Zhijian and Vizeacoumar, Franco J. and Bahr, Sondra and Li, Jingjing and Warringer, Jonas and Vizeacoumar, Frederick S. and Min, Renqiang and VanderSluis, Benjamin and Bellay, Jeremy and DeVit, Michael and Fleming, James A. and Stephens, Andrew and Haase, Julian and Lin, Zhen-Yuan and Baryshnikova, Anastasia and Lu, Hong and Yan, Zhun and Jin, Ke and Barker, Sarah and Datti, Alessandro and Giaever, Guri and Nislow, Corey and Bulawa, Chris and Myers, Chad L. and Costanzo, Michael and Gingras, Anne-Claude and Zhang, Zhaolei and Blomberg, Anders and Bloom, Kerry and Andrews, Brenda and Boone, Charles}, biburl = {https://www.bibsonomy.org/bibtex/23ba2664a61c2fe224f63e7f5de0fa4c7/karthikraman}, citeulike-article-id = {9072473}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nbt.1832}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nbt.1832}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/21441928}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=21441928}, day = 27, doi = {10.1038/nbt.1832}, interhash = {afbea1d0fdb2587281eac3a0e1761f38}, intrahash = {3ba2664a61c2fe224f63e7f5de0fa4c7}, issn = {1087-0156}, journal = {Nat Biotech}, keywords = {yeast yeast-gene-dup}, month = apr, number = 4, pages = {361--367}, pmid = {21441928}, posted-at = {2011-04-29 10:11:25}, priority = {2}, publisher = {Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {Systematic exploration of essential yeast gene function with temperature-sensitive mutants}, url = {http://dx.doi.org/10.1038/nbt.1832}, volume = 29, year = 2011 }