Article,

A Continuous-flow Method For Determining Dissociation Constants By Ultraviolet Spectrophotometry

, and .
Applied Spectroscopy, 7 (4): 164-166 (November 1953)
DOI: 10.1366/000370253774634739

Abstract

The ultraviolet absorption spectra of organic compounds which have an acidic or basic functional group as an integral part of the chromophoric system will generally be pH-dependent; that is, the form and intensity of the absorption curve will change as the hydrogen ion concentration changes. This property may be utilized in the determination of dissociation constants of an acid or base by spectrophotometry.

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