%0 Journal Article %1 luijkx_relative_2003 %A Luijkx, Thomas A. %A van Dijken, Harry %A Hamstra, Hendrik-Jan %A Kuipers, Betsy %A van der Ley, Peter %A van Alphen, Loek %A van den Dobbelsteen, Germie %D 2003 %J Infection and Immunity %K imported %N 11 %P 6367–6371 %R 10.1128/IAI.71.11.6367-6371.2003 %T Relative Immunogenicity of PorA Subtypes in a Multivalent Neisseria meningitidis Vaccine Is Not Dependent on Presentation Form %U http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=219571 %V 71 %X The hexavalent meningococcal vaccine HexaMen, containing six PorAs on two vesicles, was tested in clinical studies. Although fourfold increases in serum bactericidal activity (SBA) titers against all of the PorAs were observed, there were significant differences between PorA-specific SBA titers. SBA titers were mainly directed against one PorA from each vesicle, P1.5-2,10 and P1.5-1,2-2, and were lower against the other PorAs, especially P1.7-2,4 and P1.19,15-1. We investigated whether these differences were due to immunological interference that resulted in competition between the three PorAs on the same vesicle or whether they were caused by a difference in the immunogenicities of the separate PorAs. Therefore, mice were immunized either with HexaMen, with six monovalent outer membrane vesicles (OMVs) representing the same six PorAs simultaneously (HexaMix), or with only one of the monovalent OMVs. The immunoglobulin G and SBA titers after HexaMen immunization in mice resembled the results obtained in clinical studies. Although immunization with HexaMix gave higher titers than immunization with HexaMen for some PorAs, the pattern of high and low titers was the same. Similar differences in immunogenicity between subtypes were seen after monovalent immunization when interference was eliminated as a cause of the differences. Monovalent immunization resulted in higher titers for P1.5-1,2-2 and P1.7,16 than immunization with HexaMen. However, no significant differences were found for the weakly immunogenic PorAs, P1.7-2,4 and P1.19,15-1. Since immunization with the six PorAs in the trivalent presentation form (HexaMen) and in the mixture of monovalent vesicles (HexaMix) resulted in the same pattern of high and low titers, we concluded that the differences between the PorA-specific responses are due to differences in the immunogenicities of the various PorAs and not due to interference that results in competition between different PorAs.

@article{luijkx_relative_2003, abstract = {The hexavalent meningococcal vaccine {HexaMen,} containing six {PorAs} on two vesicles, was tested in clinical studies. Although fourfold increases in serum bactericidal activity {(SBA)} titers against all of the {PorAs} were observed, there were significant differences between {PorA-specific} {SBA} titers. {SBA} titers were mainly directed against one {PorA} from each vesicle, P1.5-2,10 and P1.5-1,2-2, and were lower against the other {PorAs,} especially P1.7-2,4 and P1.19,15-1. We investigated whether these differences were due to immunological interference that resulted in competition between the three {PorAs} on the same vesicle or whether they were caused by a difference in the immunogenicities of the separate {PorAs.} Therefore, mice were immunized either with {HexaMen,} with six monovalent outer membrane vesicles {(OMVs)} representing the same six {PorAs} simultaneously {(HexaMix),} or with only one of the monovalent {OMVs.} The immunoglobulin G and {SBA} titers after {HexaMen} immunization in mice resembled the results obtained in clinical studies. Although immunization with {HexaMix} gave higher titers than immunization with {HexaMen} for some {PorAs,} the pattern of high and low titers was the same. Similar differences in immunogenicity between subtypes were seen after monovalent immunization when interference was eliminated as a cause of the differences. Monovalent immunization resulted in higher titers for P1.5-1,2-2 and P1.7,16 than immunization with {HexaMen.} However, no significant differences were found for the weakly immunogenic {PorAs,} P1.7-2,4 and P1.19,15-1. Since immunization with the six {PorAs} in the trivalent presentation form {(HexaMen)} and in the mixture of monovalent vesicles {(HexaMix)} resulted in the same pattern of high and low titers, we concluded that the differences between the {PorA-specific} responses are due to differences in the immunogenicities of the various {PorAs} and not due to interference that results in competition between different {PorAs.}}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Luijkx, Thomas A. and van Dijken, Harry and Hamstra, {Hendrik-Jan} and Kuipers, Betsy and van der Ley, Peter and van Alphen, Loek and van den Dobbelsteen, Germie}, biburl = {https://www.bibsonomy.org/bibtex/22c8f045aed518bf7bc197641b82a337e/jelias}, doi = {10.1128/IAI.71.11.6367-6371.2003}, interhash = {ba5a9d195eddc6f6918642e2068723b6}, intrahash = {2c8f045aed518bf7bc197641b82a337e}, journal = {Infection and Immunity}, keywords = {imported}, month = nov, note = {{PMC219571}}, number = 11, pages = {6367–6371}, timestamp = {2011-03-11T10:06:14.000+0100}, title = {Relative Immunogenicity of {PorA} Subtypes in a Multivalent Neisseria meningitidis Vaccine Is Not Dependent on Presentation Form}, url = {http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=219571}, volume = 71, year = 2003 }