%0 Journal Article %1 Bond_2004_H1378 %A Bondarenko, Vladimir E %A Szigeti, Gyula P %A Bett, Glenna C L %A Kim, Song-Jung %A Rasmusson, Randall L %D 2004 %J Am. J. Physiol. Heart Circ. Physiol. %K 15142845 Action Animals, Calcium Calcium, Cardiac, Cardiovascular, Channels, Chloride Computer Conductivity, Electric Function, Gov't, Homeostasis, L-Type, Mice, Models, Myocytes, Non-P.H.S., Non-U.S. P.H.S., Patch-Clamp Potassium Potentials, Research Reticulum, Sarcoplasmic Simulation, Sodium Support, Techniques, U.S. Ventricular Voltage-Gated, %N 3 %P H1378-403 %R 10.1152/ajpheart.00185.2003 %T Computer model of action potential of mouse ventricular myocytes. %U http://dx.doi.org/10.1152/ajpheart.00185.2003 %V 287 %X We have developed a mathematical model of the mouse ventricular myocyte action potential (AP) from voltage-clamp data of the underlying currents and Ca$^2+$ transients. Wherever possible, we used Markov models to represent the molecular structure and function of ion channels. The model includes detailed intracellular Ca$^2+$ dynamics, with simulations of localized events such as sarcoplasmic Ca$^2+$ release into a small intracellular volume bounded by the sarcolemma and sarcoplasmic reticulum. Transporter-mediated Ca$^2+$ fluxes from the bulk cytosol are closely matched to the experimentally reported values and predict stimulation rate-dependent changes in Ca$^2+$ transients. Our model reproduces the properties of cardiac myocytes from two different regions of the heart: the apex and the septum. The septum has a relatively prolonged AP, which reflects a relatively small contribution from the rapid transient outward K$^+$ current in the septum. The attribution of putative molecular bases for several of the component currents enables our mouse model to be used to simulate the behavior of genetically modified transgenic mice.

@article{Bond_2004_H1378, abstract = {We have developed a mathematical model of the mouse ventricular myocyte action potential (AP) from voltage-clamp data of the underlying currents and {C}a$^{2+}$ transients. Wherever possible, we used Markov models to represent the molecular structure and function of ion channels. The model includes detailed intracellular {C}a$^{2+}$ dynamics, with simulations of localized events such as sarcoplasmic {C}a$^{2+}$ release into a small intracellular volume bounded by the sarcolemma and sarcoplasmic reticulum. Transporter-mediated {C}a$^{2+}$ fluxes from the bulk cytosol are closely matched to the experimentally reported values and predict stimulation rate-dependent changes in {C}a$^{2+}$ transients. Our model reproduces the properties of cardiac myocytes from two different regions of the heart: the apex and the septum. The septum has a relatively prolonged AP, which reflects a relatively small contribution from the rapid transient outward {K}$^{+}$ current in the septum. The attribution of putative molecular bases for several of the component currents enables our mouse model to be used to simulate the behavior of genetically modified transgenic mice.}, added-at = {2009-06-03T11:20:58.000+0200}, author = {Bondarenko, Vladimir E and Szigeti, Gyula P and Bett, Glenna C L and Kim, Song-Jung and Rasmusson, Randall L}, biburl = {https://www.bibsonomy.org/bibtex/2ce4050536a67a6dbd50a7f84d18acc21/hake}, description = {The whole bibliography file I use.}, doi = {10.1152/ajpheart.00185.2003}, file = {Bond_2004_H1378.pdf:Bond_2004_H1378.pdf:PDF}, interhash = {bd4b186fa0160a8e0df9ce8e6642d5dc}, intrahash = {ce4050536a67a6dbd50a7f84d18acc21}, journal = {Am. J. Physiol. Heart Circ. Physiol.}, key = 2, keywords = {15142845 Action Animals, Calcium Calcium, Cardiac, Cardiovascular, Channels, Chloride Computer Conductivity, Electric Function, Gov't, Homeostasis, L-Type, Mice, Models, Myocytes, Non-P.H.S., Non-U.S. P.H.S., Patch-Clamp Potassium Potentials, Research Reticulum, Sarcoplasmic Simulation, Sodium Support, Techniques, U.S. Ventricular Voltage-Gated,}, month = Sep, number = 3, pages = {H1378-403}, pdf = {Bond_2004_H1378.pdf}, pii = {00185.2003}, timestamp = {2009-06-03T11:21:06.000+0200}, title = {Computer model of action potential of mouse ventricular myocytes.}, url = {http://dx.doi.org/10.1152/ajpheart.00185.2003}, volume = 287, year = 2004 }