Single-molecule localization microscopy (SMLM) aims for maximized precision and a high signal-to-noise ratio1. Both features can be provided by placing the emitter in front of a metal-dielectric nanocoating that acts as a tuned mirror2–4. Here, we demonstrate that a higher photon yield at a lower background on biocompatible metal-dielectric nanocoatings substantially improves SMLM performance and increases the localization precision by up to a factor of two. The resolution improvement relies solely on easy-to-fabricate nanocoatings on standard glass coverslips and is spectrally and spatially tunable by the layer design and wavelength, as experimentally demonstrated for dual-color SMLM in cells.
%0 Journal Article
%1 heil2018sharpening
%A Heil, Hannah S.
%A Schreiber, Benjamin
%A Götz, Ralph
%A Emmerling, Monika
%A Dabauvalle, Marie-Christine
%A Krohne, Georg
%A Höfling, Sven
%A Kamp, Martin
%A Sauer, Markus
%A Heinze, Katrin G.
%D 2018
%J Light: Science & Applications
%K sauer
%N 1
%P 99--
%R 10.1038/s41377-018-0104-z
%T Sharpening emitter localization in front of a tuned mirror
%U https://doi.org/10.1038/s41377-018-0104-z
%V 7
%X Single-molecule localization microscopy (SMLM) aims for maximized precision and a high signal-to-noise ratio1. Both features can be provided by placing the emitter in front of a metal-dielectric nanocoating that acts as a tuned mirror2–4. Here, we demonstrate that a higher photon yield at a lower background on biocompatible metal-dielectric nanocoatings substantially improves SMLM performance and increases the localization precision by up to a factor of two. The resolution improvement relies solely on easy-to-fabricate nanocoatings on standard glass coverslips and is spectrally and spatially tunable by the layer design and wavelength, as experimentally demonstrated for dual-color SMLM in cells.
@article{heil2018sharpening,
abstract = {Single-molecule localization microscopy (SMLM) aims for maximized precision and a high signal-to-noise ratio1. Both features can be provided by placing the emitter in front of a metal-dielectric nanocoating that acts as a tuned mirror2–4. Here, we demonstrate that a higher photon yield at a lower background on biocompatible metal-dielectric nanocoatings substantially improves SMLM performance and increases the localization precision by up to a factor of two. The resolution improvement relies solely on easy-to-fabricate nanocoatings on standard glass coverslips and is spectrally and spatially tunable by the layer design and wavelength, as experimentally demonstrated for dual-color SMLM in cells.},
added-at = {2018-12-05T08:56:30.000+0100},
author = {Heil, Hannah S. and Schreiber, Benjamin and Götz, Ralph and Emmerling, Monika and Dabauvalle, Marie-Christine and Krohne, Georg and Höfling, Sven and Kamp, Martin and Sauer, Markus and Heinze, Katrin G.},
biburl = {https://www.bibsonomy.org/bibtex/23915fd80ae604d5feac1e3cf11f9053f/reichert},
doi = {10.1038/s41377-018-0104-z},
interhash = {d0f54dac3f9b0e66616058a701c57528},
intrahash = {3915fd80ae604d5feac1e3cf11f9053f},
issn = {20477538},
journal = {Light: Science & Applications},
keywords = {sauer},
number = 1,
pages = {99--},
refid = {Heil2018},
timestamp = {2018-12-05T08:56:30.000+0100},
title = {Sharpening emitter localization in front of a tuned mirror},
url = {https://doi.org/10.1038/s41377-018-0104-z},
volume = 7,
year = 2018
}