%0 Journal Article %1 Lohse2008a %A Lohse, M. J. %A Hein, P. %A Hoffmann, C. %A Nikolaev, V. O. %A Vilardaga, J. P. %A Bunemann, M. %D 2008 %J Br J Pharmacol %K & Animals Arrestins/metabolism G-Protein-Coupled G-Protein-Coupled/agonists/antagonists GTP-Binding Humans Kinases/metabolism Kinetics Messenger Proteins/metabolism/physiology Receptor Second Signal Systems/drug Transduction/drug effects/*physiology effects/physiology inhibitors/drug %P S125-32 %T Kinetics of G-protein-coupled receptor signals in intact cells %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18193071 %V 153 Suppl 1 %X G-protein-coupled receptors (GPCRs) are the largest group of cell surface receptors. They are stimulated by a variety of stimuli and signal to different classes of effectors, including several types of ion channels and second messenger-generating enzymes. Recent technical advances, most importantly in the optical recording with energy transfer techniques--fluorescence and bioluminescence resonance energy transfer, FRET and BRET--, have permitted a detailed kinetic analysis of the individual steps of the signalling chain, ranging from ligand binding to the production of second messengers in intact cells. The transfer of information, which is initiated by ligand binding, triggers a signalling cascade that displays various rate-controlling steps at different levels. This review summarizes recent findings illustrating the speed and the complexity of this signalling system.

@article{Lohse2008a, abstract = {G-protein-coupled receptors (GPCRs) are the largest group of cell surface receptors. They are stimulated by a variety of stimuli and signal to different classes of effectors, including several types of ion channels and second messenger-generating enzymes. Recent technical advances, most importantly in the optical recording with energy transfer techniques--fluorescence and bioluminescence resonance energy transfer, FRET and BRET--, have permitted a detailed kinetic analysis of the individual steps of the signalling chain, ranging from ligand binding to the production of second messengers in intact cells. The transfer of information, which is initiated by ligand binding, triggers a signalling cascade that displays various rate-controlling steps at different levels. This review summarizes recent findings illustrating the speed and the complexity of this signalling system.}, added-at = {2010-12-14T18:12:02.000+0100}, author = {Lohse, M. J. and Hein, P. and Hoffmann, C. and Nikolaev, V. O. and Vilardaga, J. P. and Bunemann, M.}, biburl = {https://www.bibsonomy.org/bibtex/25ebbefef8bf9c7ead62c5c7412321e8d/pharmawuerz}, endnotereftype = {Journal Article}, interhash = {eed5a355f5a933a109d68937c3429247}, intrahash = {5ebbefef8bf9c7ead62c5c7412321e8d}, issn = {0007-1188 (Print) 0007-1188 (Linking)}, journal = {Br J Pharmacol}, keywords = {& Animals Arrestins/metabolism G-Protein-Coupled G-Protein-Coupled/agonists/antagonists GTP-Binding Humans Kinases/metabolism Kinetics Messenger Proteins/metabolism/physiology Receptor Second Signal Systems/drug Transduction/drug effects/*physiology effects/physiology inhibitors/drug}, month = Mar, note = {Lohse, M J Hein, P Hoffmann, C Nikolaev, V O Vilardaga, J-P Bunemann, M Research Support, Non-U.S. Gov't Review England British journal of pharmacology Br J Pharmacol. 2008 Mar;153 Suppl 1:S125-32. Epub 2008 Jan 14.}, pages = {S125-32}, shorttitle = {Kinetics of G-protein-coupled receptor signals in intact cells}, timestamp = {2010-12-14T18:20:06.000+0100}, title = {Kinetics of G-protein-coupled receptor signals in intact cells}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18193071}, volume = {153 Suppl 1}, year = 2008 }