Abstract
Karyotyping is a process in which chromosomes in a dividing cell are properly stained, identified and
displayed in a standard format, which helps geneticist to study and diagnose genetic factors behind various
genetic diseases and for studying cancer. M-FISH (Multiplex Fluorescent In-Situ Hybridization) provides
color karyotyping. In this paper, an automated method for M-FISH chromosome segmentation based on
watershed transform followed by naive Bayes classification of each region using the features, mean and
standard deviation, is presented. Also, a post processing step is added to re-classify the small chromosome
segments to the neighboring larger segment for reducing the chances of misclassification. The approach
provided improved accuracy when compared to the pixel-by-pixel approach. The approach was tested on
40 images from the dataset and achieved an accuracy of 84.21 %.
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