%0 Journal Article %1 citeulike:488365 %A Heineman, W. R. %A Anderson, C. W. %A Halsall, H. B. %D 1979 %J Science %K labeled_mediator immunoassay homogeneous immunosensor ag_detection electrochemistry %N 4395 %P 865--866 %T Immunoassay by differential pulse polarography. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=441741 %V 204 %X An immunological method based on labeling an antigen with an electroactive group detectable by differential pulse polarography has been demonstrated. Estriol labeled with mercuric acetate is electroactive, giving a reduction wave at -300 millivolts versus a standard calomel electrode. Addition of estriol antibody to 4-mercuric acetate estriol diminishes the peak current as a result of the antigen-antibody binding reaction. Separation of free-labeled estriol from antibody-bound-labeled antigen is unnecessary. The method is potentially useful as an analytical immunological technique.

@article{citeulike:488365, abstract = {An immunological method based on labeling an antigen with an electroactive group detectable by differential pulse polarography has been demonstrated. Estriol labeled with mercuric acetate is electroactive, giving a reduction wave at -300 millivolts versus a standard calomel electrode. Addition of estriol antibody to 4-mercuric acetate estriol diminishes the peak current as a result of the antigen-antibody binding reaction. Separation of free-labeled estriol from antibody-bound-labeled antigen is unnecessary. The method is potentially useful as an analytical immunological technique.}, added-at = {2006-07-07T01:10:50.000+0200}, author = {Heineman, W. R. and Anderson, C. W. and Halsall, H. B.}, biburl = {https://www.bibsonomy.org/bibtex/21eed5cfd9a3de724df929983301b2ba1/biblio24}, citeulike-article-id = {488365}, interhash = {f24423fb01a18df0019bc63e00461e77}, intrahash = {1eed5cfd9a3de724df929983301b2ba1}, issn = {0036-8075}, journal = {Science}, keywords = {labeled_mediator immunoassay homogeneous immunosensor ag_detection electrochemistry}, month = May, number = 4395, pages = {865--866}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Immunoassay by differential pulse polarography.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=441741}, volume = 204, year = 1979 }