Reconstructing gene regulatory networks from high-throughput data is a long-standing challenge. Through the Dialogue on Reverse Engineering Assessment and Methods (DREAM) project, we performed a comprehensive blind assessment of over 30 network inference methods on Escherichia coli, Staphylococcus aureus, Saccharomyces cerevisiae and in silico microarray data. We characterize the performance, data requirements and inherent biases of different inference approaches, and we provide guidelines for algorithm application and development. We observed that no single inference method performs optimally across all data sets. In contrast, integration of predictions from multiple inference methods shows robust and high performance across diverse data sets. We thereby constructed high-confidence networks for E. coli and S. aureus, each comprising \~1,700 transcriptional interactions at a precision of \~50%. We experimentally tested 53 previously unobserved regulatory interactions in E. coli, of which 23 (43%) were supported. Our results establish community-based methods as a powerful and robust tool for the inference of transcriptional gene regulatory networks.
Description
Wisdom of crowds for robust gene network inference
%0 Journal Article
%1 Marbach2012Wisdom
%A Marbach, D
%A Costello, J C
%A Küffner, R
%A Vega, N M
%A Prill, R J
%A Camacho, D M
%A Allison, K R
%A DREAM5 Consortium,
%A Kellis, M
%A Collins, J J
%A Stolovitzky, G
%D 2012
%J Nat Methods
%K DREAM gene-regulatory-networks inference
%N 8
%P 796-804
%R 10.1038/nmeth.2016
%T Wisdom of crowds for robust gene network inference
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3512113/
%V 9
%X Reconstructing gene regulatory networks from high-throughput data is a long-standing challenge. Through the Dialogue on Reverse Engineering Assessment and Methods (DREAM) project, we performed a comprehensive blind assessment of over 30 network inference methods on Escherichia coli, Staphylococcus aureus, Saccharomyces cerevisiae and in silico microarray data. We characterize the performance, data requirements and inherent biases of different inference approaches, and we provide guidelines for algorithm application and development. We observed that no single inference method performs optimally across all data sets. In contrast, integration of predictions from multiple inference methods shows robust and high performance across diverse data sets. We thereby constructed high-confidence networks for E. coli and S. aureus, each comprising \~1,700 transcriptional interactions at a precision of \~50%. We experimentally tested 53 previously unobserved regulatory interactions in E. coli, of which 23 (43%) were supported. Our results establish community-based methods as a powerful and robust tool for the inference of transcriptional gene regulatory networks.
@article{Marbach2012Wisdom,
abstract = {Reconstructing gene regulatory networks from high-throughput data is a long-standing challenge. Through the Dialogue on Reverse Engineering Assessment and Methods (DREAM) project, we performed a comprehensive blind assessment of over 30 network inference methods on Escherichia coli, Staphylococcus aureus, Saccharomyces cerevisiae and in silico microarray data. We characterize the performance, data requirements and inherent biases of different inference approaches, and we provide guidelines for algorithm application and development. We observed that no single inference method performs optimally across all data sets. In contrast, integration of predictions from multiple inference methods shows robust and high performance across diverse data sets. We thereby constructed high-confidence networks for E. coli and S. aureus, each comprising \~1,700 transcriptional interactions at a precision of \~50%. We experimentally tested 53 previously unobserved regulatory interactions in E. coli, of which 23 (43%) were supported. Our results establish community-based methods as a powerful and robust tool for the inference of transcriptional gene regulatory networks.},
added-at = {2019-10-05T07:25:31.000+0200},
author = {Marbach, D and Costello, J C and K{\"u}ffner, R and Vega, N M and Prill, R J and Camacho, D M and Allison, K R and {DREAM5 Consortium} and Kellis, M and Collins, J J and Stolovitzky, G},
biburl = {https://www.bibsonomy.org/bibtex/20c358fc463d443fe618a50a41f2ddc0e/karthikraman},
description = {Wisdom of crowds for robust gene network inference},
doi = {10.1038/nmeth.2016},
interhash = {adb4a632e4742d6fc5142d330b5d8133},
intrahash = {0c358fc463d443fe618a50a41f2ddc0e},
journal = {Nat Methods},
keywords = {DREAM gene-regulatory-networks inference},
month = jul,
number = 8,
pages = {796-804},
pmid = {22796662},
timestamp = {2019-10-05T07:25:31.000+0200},
title = {Wisdom of crowds for robust gene network inference},
url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3512113/},
volume = 9,
year = 2012
}