%0 Journal Article %1 Druwe2016 %A Druwe, Ingrid %A Freudenrich, Theresa M. %A Wallace, Kathleen %A Shafer, Timothy J. %A Mundy, William R. %D 2016 %J Applied In Vitro Toxicology %K imported %R 10.1089/aivt.2015.0025 %T Comparison of Human Induced Pluripotent Stem Cell-Derived Neurons and Rat Primary Cortical Neurons as In Vitro Models of Neurite Outgrowth %X Abstract High-throughput, high-content imaging (HCI) assays for the key cellular events of neurodevelopment have been proposed to rapidly evaluate chemicals for developmental neurotoxicity. Thus, in vitro assays using neural cell cultures are being developed for key neurodevelopmental events, including proliferation, neural differentiation, migration, synaptogenesis, and neurite outgrowth. The advent of human induced pluripotent stem cell (iPSC)-derived neurons provides a novel model system. Human iPSC-derived neurons demonstrate the morphology and physiology of their in situ counterparts and have been commercialized and made available in large numbers, facilitating their use in chemical testing. The present study evaluated the neuronal characteristics of commercially available, cryopreserved human iPSC-derived neurons (iCell® neurons). Immunocytochemistry revealed a relatively pure population of neurons that extended both putative axons and dendrites. The cells developed neurites rapidly and consistently...

@article{Druwe2016, abstract = {Abstract High-throughput, high-content imaging (HCI) assays for the key cellular events of neurodevelopment have been proposed to rapidly evaluate chemicals for developmental neurotoxicity. Thus, in vitro assays using neural cell cultures are being developed for key neurodevelopmental events, including proliferation, neural differentiation, migration, synaptogenesis, and neurite outgrowth. The advent of human induced pluripotent stem cell (iPSC)-derived neurons provides a novel model system. Human iPSC-derived neurons demonstrate the morphology and physiology of their in situ counterparts and have been commercialized and made available in large numbers, facilitating their use in chemical testing. The present study evaluated the neuronal characteristics of commercially available, cryopreserved human iPSC-derived neurons (iCell{\textregistered} neurons). Immunocytochemistry revealed a relatively pure population of neurons that extended both putative axons and dendrites. The cells developed neurites rapidly and consistently...}, added-at = {2019-06-18T22:58:43.000+0200}, author = {Druwe, Ingrid and Freudenrich, Theresa M. and Wallace, Kathleen and Shafer, Timothy J. and Mundy, William R.}, biburl = {https://www.bibsonomy.org/bibtex/20e9f136cc8aa5a933e6cac93cc44950d/fcdi}, doi = {10.1089/aivt.2015.0025}, interhash = {be6ba8a7cab7d7f813cba6c85adbb378}, intrahash = {0e9f136cc8aa5a933e6cac93cc44950d}, issn = {2332-1512}, journal = {Applied In Vitro Toxicology}, keywords = {imported}, timestamp = {2019-06-18T22:58:43.000+0200}, title = {{ Comparison of Human Induced Pluripotent Stem Cell-Derived Neurons and Rat Primary Cortical Neurons as In Vitro Models of Neurite Outgrowth }}, year = 2016 }