Altered cardiac workload has an important effect on myocyte structure
and function. Cardiac hypertrophy resulting from an increase in load
has been studied extensively in the past. However, the effects of
unloading and atrophy have recently become of more interest since
devices for mechanical left ventricular unloading have been introduced
into clinical practice for the treatment of patients with terminal
heart failure, and a resulting improved cardiac and myocyte contractility
have been reported. We used the heterotopic abdominal mouse heart
transplant model in order to study the effects of 5 days of unloading
on cell size (confocal microscopy), contractility (fractional shortening:
video motion), calcium homeostasis (Ca$^2+$(i)transients, SR
Ca$^2+$content); and L-type Ca$^2+$and sodium/calcium exchanger
currents (whole cell patch clamp technique). We found unloading caused
decreased cell volume consistent with atrophy. An increased fractional
shortening and Ca$^2+$(i)transient were observed in myocytes
from unloaded hearts as compared with controls. Transsarcolemmal
I(Ca,L)and I(Na/Ca)densities, and SR Ca$^2+$content were unaltered,
as was membrane capacitance. A reduction in cell volume with mainteinance
of internal and surface membrane areas, and/or a decrease in concentration
of cellular protein Ca$^2+$buffers, may contribute to the increase
in the Ca$^2+$(i)transient in this model.
%0 Journal Article
%1 Ritt_2000_577
%A Ritter, M.
%A Su, Z.
%A Xu, S.
%A Shelby, J.
%A Barry, W. H.
%D 2000
%J J. Mol. Cell. Cardiol.
%K 10756115 ATPase, Animal, Animals, Aortic BALB C, Calcium Calcium, Cardiac Cardiomegaly, Cells, Channels, Contraction, Cultured, Disease Diseases, Dysfunction, Exchanger, Female, Gov't, Heart Heart, Homeostasis, Humans, Inbred L-Type, Left, Low, Male, Mice, Models, Muscle Muscles, Myocardial Myocardium, Non-P.H.S., Non-U.S. Output, P.H.S., Research Reticulum, Sarcoplasmic Sodium, Sodium-Calcium Stenosis, Support, U.S. Valve Ventricles, Ventricular {C}a$^{2+}$-Transporting
%N 4
%P 577-84
%R 10.1006/jmcc.2000.1101
%T Cardiac unloading alters contractility and calcium homeostasis in
ventricular myocytes.
%U http://dx.doi.org/10.1006/jmcc.2000.1101
%V 32
%X Altered cardiac workload has an important effect on myocyte structure
and function. Cardiac hypertrophy resulting from an increase in load
has been studied extensively in the past. However, the effects of
unloading and atrophy have recently become of more interest since
devices for mechanical left ventricular unloading have been introduced
into clinical practice for the treatment of patients with terminal
heart failure, and a resulting improved cardiac and myocyte contractility
have been reported. We used the heterotopic abdominal mouse heart
transplant model in order to study the effects of 5 days of unloading
on cell size (confocal microscopy), contractility (fractional shortening:
video motion), calcium homeostasis (Ca$^2+$(i)transients, SR
Ca$^2+$content); and L-type Ca$^2+$and sodium/calcium exchanger
currents (whole cell patch clamp technique). We found unloading caused
decreased cell volume consistent with atrophy. An increased fractional
shortening and Ca$^2+$(i)transient were observed in myocytes
from unloaded hearts as compared with controls. Transsarcolemmal
I(Ca,L)and I(Na/Ca)densities, and SR Ca$^2+$content were unaltered,
as was membrane capacitance. A reduction in cell volume with mainteinance
of internal and surface membrane areas, and/or a decrease in concentration
of cellular protein Ca$^2+$buffers, may contribute to the increase
in the Ca$^2+$(i)transient in this model.
@article{Ritt_2000_577,
abstract = {Altered cardiac workload has an important effect on myocyte structure
and function. Cardiac hypertrophy resulting from an increase in load
has been studied extensively in the past. However, the effects of
unloading and atrophy have recently become of more interest since
devices for mechanical left ventricular unloading have been introduced
into clinical practice for the treatment of patients with terminal
heart failure, and a resulting improved cardiac and myocyte contractility
have been reported. We used the heterotopic abdominal mouse heart
transplant model in order to study the effects of 5 days of unloading
on cell size (confocal microscopy), contractility (fractional shortening:
video motion), calcium homeostasis ([{C}a$^{2+}$](i)transients, SR
{C}a$^{2+}$content); and L-type {C}a$^{2+}$and sodium/calcium exchanger
currents (whole cell patch clamp technique). We found unloading caused
decreased cell volume consistent with atrophy. An increased fractional
shortening and [{C}a$^{2+}$](i)transient were observed in myocytes
from unloaded hearts as compared with controls. Transsarcolemmal
I(Ca,L)and I(Na/Ca)densities, and SR {C}a$^{2+}$content were unaltered,
as was membrane capacitance. A reduction in cell volume with mainteinance
of internal and surface membrane areas, and/or a decrease in concentration
of cellular protein {C}a$^{2+}$buffers, may contribute to the increase
in the [{C}a$^{2+}$](i)transient in this model.},
added-at = {2009-06-03T11:20:58.000+0200},
author = {Ritter, M. and Su, Z. and Xu, S. and Shelby, J. and Barry, W. H.},
biburl = {https://www.bibsonomy.org/bibtex/21d798b6fe6458dad3b8701b8c115cfcd/hake},
description = {The whole bibliography file I use.},
doi = {10.1006/jmcc.2000.1101},
file = {Ritt_2000_577.pdf:Ritt_2000_577.pdf:PDF},
interhash = {52a4d4872913eb7b973a5d1d0d4ff021},
intrahash = {1d798b6fe6458dad3b8701b8c115cfcd},
journal = {J. Mol. Cell. Cardiol.},
keywords = {10756115 ATPase, Animal, Animals, Aortic BALB C, Calcium Calcium, Cardiac Cardiomegaly, Cells, Channels, Contraction, Cultured, Disease Diseases, Dysfunction, Exchanger, Female, Gov't, Heart Heart, Homeostasis, Humans, Inbred L-Type, Left, Low, Male, Mice, Models, Muscle Muscles, Myocardial Myocardium, Non-P.H.S., Non-U.S. Output, P.H.S., Research Reticulum, Sarcoplasmic Sodium, Sodium-Calcium Stenosis, Support, U.S. Valve Ventricles, Ventricular {C}a$^{2+}$-Transporting},
month = Apr,
number = 4,
pages = {577-84},
pii = {S0022282800911015},
timestamp = {2009-06-03T11:21:27.000+0200},
title = {Cardiac unloading alters contractility and calcium homeostasis in
ventricular myocytes.},
url = {http://dx.doi.org/10.1006/jmcc.2000.1101},
volume = 32,
year = 2000
}