%0 Journal Article %1 citeulike:489599 %A Bojanovski, M. %A Gregg, R. E. %A Wilson, D. M. %A Brewer, H. B. %C Medizinische Hochschule Hannover, Department of Biochemistry, FRG. %D 1987 %J Clinica Chimica Acta %K elisa apob %N 2-3 %P 271--280 %R 10.1016/0009-8981(87)90137-9 %T Semi-automated enzyme-linked immunosorbent assay (ELISA) for the quantification of apolipoprotein B using monoclonal antibodies. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3436061 %V 170 %X A semi-automated competitive, double-antibody, solid-phase enzyme-linked immunosorbent assay for apolipoprotein B (Apo B) has been developed which utilizes microtiter plates with commercially available monoclonal antibodies and alkaline phosphatase-conjugated second antibody. The working range of the assay is 20-200 ng. The concentration of plasma Apo B was 0.88 +/- 0.20 g/l (n = 40) for a random sample of normal adults. The correlation coefficient for this assay, compared to a radial immunodiffusion assay, was 0.95 (slope = 1.13, intercept = -15). The quantification of the samples was not influenced by freezing and thawing, storage at -20 degrees C for up to 9 mth, or the lipoprotein particle on which the Apo B was present. The method is suitable for measurement of apolipoprotein B in either normal or pathological plasma, lipoprotein density classes, and is sensitive enough to quantify Apo B in cell biological and molecular biological investigations.

@article{citeulike:489599, abstract = {A semi-automated competitive, double-antibody, solid-phase enzyme-linked immunosorbent assay for apolipoprotein B (Apo B) has been developed which utilizes microtiter plates with commercially available monoclonal antibodies and alkaline phosphatase-conjugated second antibody. The working range of the assay is 20-200 ng. The concentration of plasma Apo B was 0.88 +/- 0.20 g/l (n = 40) for a random sample of normal adults. The correlation coefficient for this assay, compared to a radial immunodiffusion assay, was 0.95 (slope = 1.13, intercept = -15). The quantification of the samples was not influenced by freezing and thawing, storage at -20 degrees C for up to 9 mth, or the lipoprotein particle on which the Apo B was present. The method is suitable for measurement of apolipoprotein B in either normal or pathological plasma, lipoprotein density classes, and is sensitive enough to quantify Apo B in cell biological and molecular biological investigations.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Medizinische Hochschule Hannover, Department of Biochemistry, FRG.}, author = {Bojanovski, M. and Gregg, R. E. and Wilson, D. M. and Brewer, H. B.}, biburl = {https://www.bibsonomy.org/bibtex/22680b431bc0a9661ed654136e47c8b26/biblio24}, citeulike-article-id = {489599}, doi = {10.1016/0009-8981(87)90137-9}, interhash = {9521d32dcc2591af2235ceb398bbe42b}, intrahash = {2680b431bc0a9661ed654136e47c8b26}, issn = {0009-8981}, journal = {Clinica Chimica Acta}, keywords = {elisa apob}, month = {December}, number = {2-3}, pages = {271--280}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Semi-automated enzyme-linked immunosorbent assay (ELISA) for the quantification of apolipoprotein B using monoclonal antibodies.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=3436061}, volume = 170, year = 1987 }