%0 Journal Article %1 Menzel:2007:Mech-Dev:17097274 %A Menzel, N %A Schneeberger, D %A Raabe, T %D 2007 %J Mech Dev %K Mbt eye %N 1 %P 78-90 %R 10.1016/j.mod.2006.09.007 %T The Drosophila p21 activated kinase Mbt regulates the actin cytoskeleton and adherens junctions to control photoreceptor cell morphogenesis %U https://www.ncbi.nlm.nih.gov/pubmed/17097274?dopt=Citation %V 124 %X The p21 activated kinase (Pak) family of protein kinases are involved in many cellular functions like re-organisation of the cytoskeleton, transcriptional control, cell division, and survival. These pleiotropic actions are reflected in a plethora of known interacting proteins and phosphorylation substrates. Yet, the integration of a single Pak protein into signalling pathways controlling a particular developmental process are less well studied. For two of the three known Pak proteins in Drosophila melanogaster, D-Pak and Mbt, distinct functions during eye development have been established. In this study we undertook a genetic approach to identify proteins acting in the Mbt signalling pathway during photoreceptor cell morphogenesis. The genetic screen identified the actin depolymerisation factor Twinstar/Cofilin as one target of Mbt signalling. Twinstar/Cofilin becomes phosphorylated upon activation of Mbt. However, biochemical and genetic experiments question the role of the LIM domain protein kinase (Limk) as a major link between Mbt and Twinstar/Cofilin as it has been suggested for other PAK proteins. Constitutive activation of Mbt not only disturbs the actin cytoskeleton but also affects adherens junction organisation indicating a requirement of the protein in cell adhesion dependent processes during photoreceptor cell differentiation.

@article{Menzel:2007:Mech-Dev:17097274, abstract = {The p21 activated kinase (Pak) family of protein kinases are involved in many cellular functions like re-organisation of the cytoskeleton, transcriptional control, cell division, and survival. These pleiotropic actions are reflected in a plethora of known interacting proteins and phosphorylation substrates. Yet, the integration of a single Pak protein into signalling pathways controlling a particular developmental process are less well studied. For two of the three known Pak proteins in Drosophila melanogaster, D-Pak and Mbt, distinct functions during eye development have been established. In this study we undertook a genetic approach to identify proteins acting in the Mbt signalling pathway during photoreceptor cell morphogenesis. The genetic screen identified the actin depolymerisation factor Twinstar/Cofilin as one target of Mbt signalling. Twinstar/Cofilin becomes phosphorylated upon activation of Mbt. However, biochemical and genetic experiments question the role of the LIM domain protein kinase (Limk) as a major link between Mbt and Twinstar/Cofilin as it has been suggested for other PAK proteins. Constitutive activation of Mbt not only disturbs the actin cytoskeleton but also affects adherens junction organisation indicating a requirement of the protein in cell adhesion dependent processes during photoreceptor cell differentiation.}, added-at = {2019-07-24T13:27:25.000+0200}, author = {Menzel, N and Schneeberger, D and Raabe, T}, biburl = {https://www.bibsonomy.org/bibtex/23a18c363aa7849d32a502670be3e3577/steffip}, description = {The Drosophila p21 activated kinase Mbt regulates the actin cytoskeleton and adherens junctions to control photoreceptor cell morphogenesis. - PubMed - NCBI}, doi = {10.1016/j.mod.2006.09.007}, interhash = {0338b2933c7a007f4811ac563a1533e4}, intrahash = {3a18c363aa7849d32a502670be3e3577}, journal = {Mech Dev}, keywords = {Mbt eye}, month = jan, number = 1, pages = {78-90}, pmid = {17097274}, timestamp = {2019-07-24T13:27:25.000+0200}, title = {The Drosophila p21 activated kinase Mbt regulates the actin cytoskeleton and adherens junctions to control photoreceptor cell morphogenesis}, url = {https://www.ncbi.nlm.nih.gov/pubmed/17097274?dopt=Citation}, volume = 124, year = 2007 }