Aqueous mixtures of purified mucin and alginate have been shown to
form weak viscoelastic gels under appropriate conditions. These mixed
gels of mucin and alginate have been studied by both small deformation
and large deformation rheology, and the effects of temperature and
ionic strength on small deformation rheology have been investigated.
The gels were theologically reversible, flowing as the shear stress
was increased and recovering their solid dominant properties as the
shear stress decreased, and thermally stable showing little change
in small deformation rheology in the range 10-60 degreesC. The effect
of ionic strength on gel rheology was indicative of the presence
of electrostatic interactions within the gel matrix. Based on these
investigations, we propose that mixed mucin alginate gels are maintained
by both heteropolymeric mucin-alginate interactions and homopolymeric
mucin-mucin interactions. The ability of alginate to interact with
mucin and promote mucin-mucin interactions may also have relevance
to understanding the clinical problems associated with pulmonary
infection with the alginate secreting bacterium Pseudomonas aeruginosa
in cystic fibrosis patients. (C) 2004 Elsevier Ltd. All rights reserved.
%0 Journal Article
%1 Taylor2005
%A Taylor, C.
%A Pearson, J. P.
%A Draget, K. I.
%A Dettmar, P. W.
%A Smidsrod, O.
%C THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
%D 2005
%I ELSEVIER SCI LTD
%J Carbohydr. Polym.
%K ; ;; [ISI:] bioploymer; gastric in-vitro; mucoadhesion; mucus mucus; sputum; systems
%N 2
%P 189 -- 195
%T Rheological characterisation of mixed gels of mucin and alginate
%V 59
%X Aqueous mixtures of purified mucin and alginate have been shown to
form weak viscoelastic gels under appropriate conditions. These mixed
gels of mucin and alginate have been studied by both small deformation
and large deformation rheology, and the effects of temperature and
ionic strength on small deformation rheology have been investigated.
The gels were theologically reversible, flowing as the shear stress
was increased and recovering their solid dominant properties as the
shear stress decreased, and thermally stable showing little change
in small deformation rheology in the range 10-60 degreesC. The effect
of ionic strength on gel rheology was indicative of the presence
of electrostatic interactions within the gel matrix. Based on these
investigations, we propose that mixed mucin alginate gels are maintained
by both heteropolymeric mucin-alginate interactions and homopolymeric
mucin-mucin interactions. The ability of alginate to interact with
mucin and promote mucin-mucin interactions may also have relevance
to understanding the clinical problems associated with pulmonary
infection with the alginate secreting bacterium Pseudomonas aeruginosa
in cystic fibrosis patients. (C) 2004 Elsevier Ltd. All rights reserved.
@article{Taylor2005,
__markedentry = {[phpts:6]},
abstract = {Aqueous mixtures of purified mucin and alginate have been shown to
form weak viscoelastic gels under appropriate conditions. These mixed
gels of mucin and alginate have been studied by both small deformation
and large deformation rheology, and the effects of temperature and
ionic strength on small deformation rheology have been investigated.
The gels were theologically reversible, flowing as the shear stress
was increased and recovering their solid dominant properties as the
shear stress decreased, and thermally stable showing little change
in small deformation rheology in the range 10-60 degreesC. The effect
of ionic strength on gel rheology was indicative of the presence
of electrostatic interactions within the gel matrix. Based on these
investigations, we propose that mixed mucin alginate gels are maintained
by both heteropolymeric mucin-alginate interactions and homopolymeric
mucin-mucin interactions. The ability of alginate to interact with
mucin and promote mucin-mucin interactions may also have relevance
to understanding the clinical problems associated with pulmonary
infection with the alginate secreting bacterium Pseudomonas aeruginosa
in cystic fibrosis patients. (C) 2004 Elsevier Ltd. All rights reserved.},
added-at = {2011-11-04T13:47:04.000+0100},
address = {THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND},
author = {Taylor, C. and Pearson, J. P. and Draget, K. I. and Dettmar, P. W. and Smidsrod, O.},
authoraddress = {NTNU, Inst Bioteknol, N-7491 Trondheim, Norway. ; Univ Newcastle Upon
Tyne, Sch Med, Sch Cell & Mol Biosci, Newcastle Upon Tyne NE2 4HH,
Tyne & Wear, England. ; Reckitt Benckiser Healthcare UK Ltd, Kingston
Upon Hull, N Humberside, England.},
biburl = {https://www.bibsonomy.org/bibtex/23d3cfa671b24f893deefb3e17b5a5c76/pawelsikorski},
citedref = {ALLEN A, 1989, HDB PHYSL GASTROINTE, P111 ; BELL AE, 1984, INT J BIOL
MACROMOL, V6, P309 ; BROWNLEE IA, 2002, J GASTROEN HEPATOL, V17,
A991 ; CEULEMANS J, 2002, J PHARM SCI, V91, P1117 ; DASGUPTA B, 1998,
PEDIATR PULM, V26, P250 ; DESSEYN JL, 2000, MOL BIOL EVOL, V17, P1175
; FOGG FJJ, 1996, BIOCHEM J 3, V316, P937 ; FUONGFUCHAT A, 1996,
CARBOHYD RES, V284, P85 ; GENDLER SJ, 1995, ANNU REV PHYSIOL, V57,
P607 ; HAGERSTROM H, 2003, EUR J PHARM SCI, V18, P349 ; HARDING SE,
1995, BIOPHYS CHEM, V55, P69 ; HAUG A, 1967, ACTA CHEM SCAND, V21,
P691 ; JONES DS, 2003, J PHARM SCI, V92, P995 ; KAVANAGH GM, 1998,
PROG POLYM SCI, V23, P533 ; KING M, 1997, AM J RESP CRIT CARE, V156,
P173 ; KOCEVARNARED J, 1997, BIOMATERIALS, V18, P677 ; MADSEN F,
1998, BIOMATERIALS, V19, P1083 ; MOE S, 1995, FOOD POLYSACCHARIDES
; MRSNY RJ, 1994, PULM PHARMACOL, V7, P357 ; NEISER S, 1999, FOOD
HYDROCOLLOID, V13, P445 ; PATEL MM, 2003, BIOMACROMOLECULES, V4,
P1184 ; PEREZVILAR J, 1999, J BIOL CHEM, V274, P31751 ; RAYNAL BDE,
2003, J BIOL CHEM, V278, P28703 ; RILEY RG, 2001, INT J PHARM, V217,
P87 ; ROSSI S, 2000, EUR J PHARM SCI, V10, P251 ; SKAUGRUD O, 1995,
EXCIPIENTS DEL SYSTE, P96 ; SMIDSROD O, 1996, CARBOHYDRATES EUROPE,
V14, P7 ; TAYLOR C, 2003, BIOMACROMOLECULES, V4, P922},
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intrahash = {3d3cfa671b24f893deefb3e17b5a5c76},
isifile-dt = {Article},
isifile-em = {ctaylor@biotech.ntnu.no},
isifile-ga = {891IM},
isifile-j9 = {CARBOHYD POLYM},
isifile-nr = {28},
isifile-pi = {OXFORD},
isifile-rp = {Taylor, C, NTNU, Inst Bioteknol, Sem Soelands Vei 6-8, N-7491 ; Trondheim,
Norway.},
isifile-sc = {Chemistry, Applied; Chemistry, Organic; Polymer Science},
isifile-tc = {1},
issn = {0144-8617},
journal = {Carbohydr. Polym.},
keywords = {; ;; [ISI:] bioploymer; gastric in-vitro; mucoadhesion; mucus mucus; sputum; systems},
language = {English},
month = {JAN 10},
number = 2,
owner = {phpts},
pages = {189 -- 195},
publisher = {ELSEVIER SCI LTD},
size = {7 p.},
sourceid = {ISI:000226574500006},
timestamp = {2011-11-04T13:47:25.000+0100},
title = {Rheological characterisation of mixed gels of mucin and alginate},
volume = 59,
year = 2005
}