%0 Journal Article %1 Belgore.2001 %A Belgore, F. M. %A Blann, A. D. %A Lip, G. Y. %D 2001 %J Clin.Sci.(Lond) %K A Aged Antibodies Assay Cardiovascular Diseases Endothelial Endothelium Enzyme-Linked Extracellular Factor Factors Female Growth Human Humans Immunoassay Immunosorbent Lymphokines Male Matrix Middle Monoclonal Proteins RANGE Receptor-1 Reproducibility Research Results Sensitivity Solubility Specificity Thrombosis Tyrosine Vascular and blood immunology methods of protein %N 5 %P 567-575 %T Measurement of free and complexed soluble vascular endothelial growth factor receptor, Flt-1, in fluid samples: development and application of two new immunoassays %U PM:11294698 %V 100 %X Vascular endothelial growth factor (VEGF) mediates endothelial cell mitogenesis and enhances vascular permeability. VEGF interacts with the endothelium via two membrane-spanning receptors, fms-like tyrosine kinase (Flt)-1 and kinase domain receptor. A soluble form of Flt-1 (sFlt-1) was isolated from endothelial cell media; however, its biological significance is still unknown, with limited data on plasma sFlt-1 levels in disease states. We have developed two new ELISAs for detecting free and VEGF-complexed sFlt-1, which were tested in accordance with standard validation and assessment methodologies employed in commercial settings. The intra-and inter-assay coefficients of variation are <5% and 10% respectively, and results are highly reproducible. Applying these ELISAs in a clinical setting, we measured levels of VEGF, free and complexed sFlt-1 in citrated plasma from 40 patients with cardiovascular disease and 40 healthy controls. Median (interquartile range) plasma levels of VEGF in

@article{Belgore.2001, abstract = {Vascular endothelial growth factor (VEGF) mediates endothelial cell mitogenesis and enhances vascular permeability. VEGF interacts with the endothelium via two membrane-spanning receptors, fms-like tyrosine kinase (Flt)-1 and kinase domain receptor. A soluble form of Flt-1 (sFlt-1) was isolated from endothelial cell media; however, its biological significance is still unknown, with limited data on plasma sFlt-1 levels in disease states. We have developed two new ELISAs for detecting free and VEGF-complexed sFlt-1, which were tested in accordance with standard validation and assessment methodologies employed in commercial settings. The intra-and inter-assay coefficients of variation are <5% and 10% respectively, and results are highly reproducible. Applying these ELISAs in a clinical setting, we measured levels of VEGF, free and complexed sFlt-1 in citrated plasma from 40 patients with cardiovascular disease and 40 healthy controls. Median (interquartile range) plasma levels of VEGF in }, added-at = {2010-02-05T11:28:39.000+0100}, author = {Belgore, F. M. and Blann, A. D. and Lip, G. Y.}, biburl = {https://www.bibsonomy.org/bibtex/241901fb48bee29ed956bfb5bbb896eaa/kanefendt}, interhash = {1cdd48f0105e0b950851bafad3e60bea}, intrahash = {41901fb48bee29ed956bfb5bbb896eaa}, journal = {Clin.Sci.(Lond)}, keywords = {A Aged Antibodies Assay Cardiovascular Diseases Endothelial Endothelium Enzyme-Linked Extracellular Factor Factors Female Growth Human Humans Immunoassay Immunosorbent Lymphokines Male Matrix Middle Monoclonal Proteins RANGE Receptor-1 Reproducibility Research Results Sensitivity Solubility Specificity Thrombosis Tyrosine Vascular and blood immunology methods of protein}, number = 5, pages = {567-575}, timestamp = {2010-02-05T11:28:40.000+0100}, title = {Measurement of free and complexed soluble vascular endothelial growth factor receptor, Flt-1, in fluid samples: development and application of two new immunoassays}, url = {PM:11294698}, volume = 100, year = 2001 }