%0 Journal Article %1 WOS:000459288100009 %A Ren, Z %A Kim, D %A Paula, A J %A Hwang, G %A Liu, Y %A Li, J %A Daniell, H %A Koo, H %C 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA %D 2019 %I SAGE PUBLICATIONS INC %J JOURNAL OF DENTAL RESEARCH %K agents; anti-infective caries; dental drug extracellular matrix; polymeric resistance; streptococcus} substance {biofilms; %N 3 %P 322-330 %R 10.1177/0022034518818480 %T Dual-Targeting Approach Degrades Biofilm Matrix and Enhances Bacterial Killing %V 98 %X Biofilm formation is a key virulence factor responsible for a wide range of infectious diseases, including dental caries. Cariogenic biofilms are structured microbial communities embedded in an extracellular matrix that affords bacterial adhesion-cohesion and drug tolerance, making them difficult to treat using conventional antimicrobial monotherapy. Here, we investigated a multitargeted approach combining exopolysaccharide (EPS) matrix-degrading glucanohydrolases with a clinically used essential oils-based antimicrobial to potentiate antibiofilm efficacy. Our data showed that dextranase and mutanase can synergistically break down the EPS glucan matrix in preformed cariogenic biofilms, markedly enhancing bacterial killing by the antimicrobial agent (3-log increase versus antimicrobial alone). Further analyses revealed that an EPS-degrading/antimicrobial (EDA) approach disassembles the matrix scaffold, exposing the bacterial cells for efficient killing while concurrently causing cellular dispersion and ``physical collapse'' of the bacterial clusters. Unexpectedly, we found that the EDA approach can also selectively target the EPS-producing cariogenic bacteria Streptococcus mutans with higher killing specificity (versus other species) within mixed biofilms, disrupting their accumulation and promoting dominance of commensal bacteria. Together, these results demonstrate a dual-targeting approach that can enhance antibiofilm efficacy and precision by dismantling the EPS matrix and its protective microenvironment, amplifying the killing of pathogenic bacteria within.

@article{WOS:000459288100009, abstract = {Biofilm formation is a key virulence factor responsible for a wide range of infectious diseases, including dental caries. Cariogenic biofilms are structured microbial communities embedded in an extracellular matrix that affords bacterial adhesion-cohesion and drug tolerance, making them difficult to treat using conventional antimicrobial monotherapy. Here, we investigated a multitargeted approach combining exopolysaccharide (EPS) matrix-degrading glucanohydrolases with a clinically used essential oils-based antimicrobial to potentiate antibiofilm efficacy. Our data showed that dextranase and mutanase can synergistically break down the EPS glucan matrix in preformed cariogenic biofilms, markedly enhancing bacterial killing by the antimicrobial agent (3-log increase versus antimicrobial alone). Further analyses revealed that an EPS-degrading/antimicrobial (EDA) approach disassembles the matrix scaffold, exposing the bacterial cells for efficient killing while concurrently causing cellular dispersion and ``physical collapse'' of the bacterial clusters. Unexpectedly, we found that the EDA approach can also selectively target the EPS-producing cariogenic bacteria Streptococcus mutans with higher killing specificity (versus other species) within mixed biofilms, disrupting their accumulation and promoting dominance of commensal bacteria. Together, these results demonstrate a dual-targeting approach that can enhance antibiofilm efficacy and precision by dismantling the EPS matrix and its protective microenvironment, amplifying the killing of pathogenic bacteria within.}, added-at = {2022-05-23T20:00:14.000+0200}, address = {2455 TELLER RD, THOUSAND OAKS, CA 91320 USA}, author = {Ren, Z and Kim, D and Paula, A J and Hwang, G and Liu, Y and Li, J and Daniell, H and Koo, H}, biburl = {https://www.bibsonomy.org/bibtex/242ce999fa0a9a8ae25c5354c7158e289/ppgfis_ufc_br}, doi = {10.1177/0022034518818480}, interhash = {d18af9346a49c24a14536ac7de4c7449}, intrahash = {42ce999fa0a9a8ae25c5354c7158e289}, issn = {0022-0345}, journal = {JOURNAL OF DENTAL RESEARCH}, keywords = {agents; anti-infective caries; dental drug extracellular matrix; polymeric resistance; streptococcus} substance {biofilms;}, number = 3, pages = {322-330}, publisher = {SAGE PUBLICATIONS INC}, pubstate = {published}, timestamp = {2022-05-23T20:00:14.000+0200}, title = {Dual-Targeting Approach Degrades Biofilm Matrix and Enhances Bacterial Killing}, tppubtype = {article}, volume = 98, year = 2019 }