The proto-oncogene c-src, the cellular homolog of the Rous sarcoma virus (RSV) transforming gene v-src, is expressed in a tissue-specific and age-dependent manner. Its physiological function, although still unknown, appears to be more closely related to differentiation processes than to proliferation processes. To obtain more information about the physiological role of the c-src gene in cells, we have studied differentiation-dependent alterations using the human HL-60 leukaemia cell line as a model system. Induction of monocytic and granulocytic differentiation of HL-60 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA) and dimethylsulfoxide (DMSO) is associated with an activation of the pp60c-src tyrosine kinase, but not with increased c-src gene expression. Control experiments exclude an interaction of TPA and DMSO themselves with the pp60c-src kinase.
%0 Journal Article
%1 Barnekow.1986
%A Barnekow, A.
%A Gessler, M.
%D 1986
%J Embo J
%K Acetate/pharmacology Activation Avian Cell Differentiation Enzyme Genes Genes;Viral Humans Induction Kinases/*biosynthesis Kinetics Leukemia;Myeloid;Acute Line Oncogene Phosphorylation Protein Proteins/*biosynthesis/genetics Proto-Oncogenes Retroviridae Sarcoma Tetradecanoylphorbol Viruses/genetics pp60(v-src)
%N 4
%P 701--705
%T Activation of the pp60c-src kinase during differentiation of monomyelocytic cells in vitro
%V 5
%X The proto-oncogene c-src, the cellular homolog of the Rous sarcoma virus (RSV) transforming gene v-src, is expressed in a tissue-specific and age-dependent manner. Its physiological function, although still unknown, appears to be more closely related to differentiation processes than to proliferation processes. To obtain more information about the physiological role of the c-src gene in cells, we have studied differentiation-dependent alterations using the human HL-60 leukaemia cell line as a model system. Induction of monocytic and granulocytic differentiation of HL-60 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA) and dimethylsulfoxide (DMSO) is associated with an activation of the pp60c-src tyrosine kinase, but not with increased c-src gene expression. Control experiments exclude an interaction of TPA and DMSO themselves with the pp60c-src kinase.
@article{Barnekow.1986,
abstract = {The proto-oncogene c-src, the cellular homolog of the Rous sarcoma virus (RSV) transforming gene v-src, is expressed in a tissue-specific and age-dependent manner. Its physiological function, although still unknown, appears to be more closely related to differentiation processes than to proliferation processes. To obtain more information about the physiological role of the c-src gene in cells, we have studied differentiation-dependent alterations using the human HL-60 leukaemia cell line as a model system. Induction of monocytic and granulocytic differentiation of HL-60 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA) and dimethylsulfoxide (DMSO) is associated with an activation of the pp60c-src tyrosine kinase, but not with increased c-src gene expression. Control experiments exclude an interaction of TPA and DMSO themselves with the pp60c-src kinase.},
added-at = {2013-01-29T13:47:26.000+0100},
author = {Barnekow, A. and Gessler, M.},
biburl = {https://www.bibsonomy.org/bibtex/2431ac7993d834ea0ab5ce41e9c32535c/ebch},
interhash = {37c200402aa2ef3c5d3966de399b09cc},
intrahash = {431ac7993d834ea0ab5ce41e9c32535c},
journal = {Embo J},
keywords = {Acetate/pharmacology Activation Avian Cell Differentiation Enzyme Genes Genes;Viral Humans Induction Kinases/*biosynthesis Kinetics Leukemia;Myeloid;Acute Line Oncogene Phosphorylation Protein Proteins/*biosynthesis/genetics Proto-Oncogenes Retroviridae Sarcoma Tetradecanoylphorbol Viruses/genetics pp60(v-src)},
number = 4,
pages = {701--705},
timestamp = {2013-01-29T13:47:39.000+0100},
title = {Activation of the pp60c-src kinase during differentiation of monomyelocytic cells in vitro},
volume = 5,
year = 1986
}