DNA sequence-based tandem repeat analysis of the clfB gene is less discriminatory than spa typing for methicillin-resistant Staphylococcus aureus
D. Harmsen, H. Claus, and U. Vogel. International Journal of Medical Microbiology: IJMM, 294 (8):
525--528(March 2005)PMID: 15790297.
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) are a major challenge for hospital hygiene. Typing by DNA sequence analysis of the repeat region of the protein A gene (spa) significantly improved typing of MRSA in the hospital setting. However, microevolution of spa repeats in epidemic clones appears to occur at a fairly slow clock rate. Therefore, DNA sequence-based methods providing additional resolution are desirable in some situations. We evaluated the use of the clfB repeat region proposed recently by others as a possible complementation to spa typing. Using epidemic MRSA isolates from two German university hospitals, we show that the clfB repeat region does not offer any additional discriminatory power.
%0 Journal Article
%1 harmsen_dna_2005
%A Harmsen, Dag
%A Claus, Heike
%A Vogel, Ulrich
%D 2005
%J International Journal of Medical Microbiology: IJMM
%K A, Adhesins, Bacterial Bacterial, Humans, Infections, Methicillin Methicillin, Protein Repeat Resistance, Sequences Staphylococcal Staphylococcus Tandem Techniques, Typing aureus, {DNA},
%N 8
%P 525--528
%T DNA sequence-based tandem repeat analysis of the clfB gene is less discriminatory than spa typing for methicillin-resistant Staphylococcus aureus
%U http://www.ncbi.nlm.nih.gov/pubmed/15790297
%V 294
%X Methicillin-resistant Staphylococcus aureus (MRSA) are a major challenge for hospital hygiene. Typing by DNA sequence analysis of the repeat region of the protein A gene (spa) significantly improved typing of MRSA in the hospital setting. However, microevolution of spa repeats in epidemic clones appears to occur at a fairly slow clock rate. Therefore, DNA sequence-based methods providing additional resolution are desirable in some situations. We evaluated the use of the clfB repeat region proposed recently by others as a possible complementation to spa typing. Using epidemic MRSA isolates from two German university hospitals, we show that the clfB repeat region does not offer any additional discriminatory power.
@article{harmsen_dna_2005,
abstract = {Methicillin-resistant Staphylococcus aureus {(MRSA)} are a major challenge for hospital hygiene. Typing by {DNA} sequence analysis of the repeat region of the protein A gene (spa) significantly improved typing of {MRSA} in the hospital setting. However, microevolution of spa repeats in epidemic clones appears to occur at a fairly slow clock rate. Therefore, {DNA} sequence-based methods providing additional resolution are desirable in some situations. We evaluated the use of the {clfB} repeat region proposed recently by others as a possible complementation to spa typing. Using epidemic {MRSA} isolates from two German university hospitals, we show that the {clfB} repeat region does not offer any additional discriminatory power.},
added-at = {2011-06-24T11:21:47.000+0200},
author = {Harmsen, Dag and Claus, Heike and Vogel, Ulrich},
biburl = {https://www.bibsonomy.org/bibtex/252279d19c848ec4089f099dea63699cb/ag_vogel},
interhash = {2799ae0a7ee5c5502ca27ade48200e0b},
intrahash = {52279d19c848ec4089f099dea63699cb},
issn = {1438-4221},
journal = {International Journal of Medical Microbiology: {IJMM}},
keywords = {A, Adhesins, Bacterial Bacterial, Humans, Infections, Methicillin Methicillin, Protein Repeat Resistance, Sequences Staphylococcal Staphylococcus Tandem Techniques, Typing aureus, {DNA},},
month = mar,
note = {{PMID:} 15790297},
number = 8,
pages = {525--528},
timestamp = {2011-06-24T11:21:51.000+0200},
title = {{DNA} sequence-based tandem repeat analysis of the {clfB} gene is less discriminatory than spa typing for methicillin-resistant Staphylococcus aureus},
url = {http://www.ncbi.nlm.nih.gov/pubmed/15790297},
volume = 294,
year = 2005
}