The interaction of cardiac Na$^+$-Ca$^2+$ exchange (NCX1)
with caveolin proteins was investigated in sarcolemmal vesicles.
Western blots of sarcolemmal vesicles revealed the presence of caveolin-1,
-2, and -3. NCX1 co-fractionated more closely with caveolin-3 than
caveolin-1 on sucrose density gradients. NCX1 has five possible caveolin-binding
motifs and NCX1 co-precipitated specifically with caveolin-3. Molecular
sieve column chromatography indicated that this co-precipitation
was not due to incomplete solubilization of lipid raft microdomains.
Cholesterol chelation in vesicles decreased NCX1 transport activity
and caveolin-3 co-precipitation. NCX1 may play a role in caveolar
transmembrane signaling in addition to its role in excitation-contraction
coupling.
%0 Journal Article
%1 Boss_2002_113
%A Bossuyt, Julie
%A Taylor, Bonnie E
%A James-Kracke, Marilyn
%A Hale, Calvin C
%D 2002
%J FEBS Lett.
%K 3; Acid Amino Animals; Binding Binding; Blotting, Cattle; Caveolae, Caveolin Caveolins, Centrifugation, Cholesterol, Chromatography, Cyclodextrins, Density Exchanger, Gel; Gradient; Motifs; Myocardium, Precipitin Protein Sarcolemma, Sites; Sodium-Calcium Tests; Western; chemistry/drug chemistry/metabolism effects/metabolism; metabolism; pharmacology;
%N 1-3
%P 113--117
%R 10.1016/S0014-5793(01)03323-3
%T Evidence for cardiac sodium-calcium exchanger association with caveolin-3.
%U http://dx.doi.org/10.1016/S0014-5793(01)03323-3
%V 511
%X The interaction of cardiac Na$^+$-Ca$^2+$ exchange (NCX1)
with caveolin proteins was investigated in sarcolemmal vesicles.
Western blots of sarcolemmal vesicles revealed the presence of caveolin-1,
-2, and -3. NCX1 co-fractionated more closely with caveolin-3 than
caveolin-1 on sucrose density gradients. NCX1 has five possible caveolin-binding
motifs and NCX1 co-precipitated specifically with caveolin-3. Molecular
sieve column chromatography indicated that this co-precipitation
was not due to incomplete solubilization of lipid raft microdomains.
Cholesterol chelation in vesicles decreased NCX1 transport activity
and caveolin-3 co-precipitation. NCX1 may play a role in caveolar
transmembrane signaling in addition to its role in excitation-contraction
coupling.
@article{Boss_2002_113,
abstract = {The interaction of cardiac {N}a$^{+}$-{C}a$^{2+}$ exchange (NCX1)
with caveolin proteins was investigated in sarcolemmal vesicles.
Western blots of sarcolemmal vesicles revealed the presence of caveolin-1,
-2, and -3. NCX1 co-fractionated more closely with caveolin-3 than
caveolin-1 on sucrose density gradients. NCX1 has five possible caveolin-binding
motifs and NCX1 co-precipitated specifically with caveolin-3. Molecular
sieve column chromatography indicated that this co-precipitation
was not due to incomplete solubilization of lipid raft microdomains.
Cholesterol chelation in vesicles decreased NCX1 transport activity
and caveolin-3 co-precipitation. NCX1 may play a role in caveolar
transmembrane signaling in addition to its role in excitation-contraction
coupling.},
added-at = {2009-06-03T11:20:58.000+0200},
author = {Bossuyt, Julie and Taylor, Bonnie E and James-Kracke, Marilyn and Hale, Calvin C},
biburl = {https://www.bibsonomy.org/bibtex/2614c6fac0a8e0ea11ac4a3f0360c56d7/hake},
description = {The whole bibliography file I use.},
doi = {10.1016/S0014-5793(01)03323-3},
file = {Boss_2002_113.pdf:Boss_2002_113.pdf:PDF},
institution = {The Dalton Cardiovascular Research Center, University of Missouri,
Columbia, MO 65211, USA.},
interhash = {dd9137953203919bbde0abcf3671c3e4},
intrahash = {614c6fac0a8e0ea11ac4a3f0360c56d7},
journal = {FEBS Lett.},
keywords = {3; Acid Amino Animals; Binding Binding; Blotting, Cattle; Caveolae, Caveolin Caveolins, Centrifugation, Cholesterol, Chromatography, Cyclodextrins, Density Exchanger, Gel; Gradient; Motifs; Myocardium, Precipitin Protein Sarcolemma, Sites; Sodium-Calcium Tests; Western; chemistry/drug chemistry/metabolism effects/metabolism; metabolism; pharmacology;},
month = Jan,
number = {1-3},
pages = {113--117},
pdf = {Boss_2002_113.pdf},
pii = {S0014579301033233},
pmid = {11821059},
timestamp = {2009-06-03T11:21:06.000+0200},
title = {Evidence for cardiac sodium-calcium exchanger association with caveolin-3.},
url = {http://dx.doi.org/10.1016/S0014-5793(01)03323-3},
volume = 511,
year = 2002
}