%0 Journal Article %1 song2014cells %A Song, Kwang Hoon %A Kwon, Keon Woo %A Choi, Jong-Cheol %A Jung, Jaehwang %A Park, YongKeun %A Suh, Kahp-Yang %A Doh, Junsang %D 2014 %I The Royal Society of Chemistry %J Integr. Biol. %K filopodia methods migration phd %P - %R 10.1039/C4IB00021H %T T cells sense biophysical cues using lamellipodia and filopodia to optimize intraluminal path finding %U http://dx.doi.org/10.1039/C4IB00021H %X Intraluminal crawling is considered to be important for extravasation of leukocytes in blood vessels, but biochemical/biophysical cues guiding the crawling of leukocytes have not been clear. Here we provide evidences that T cells sense the topography of luminal surfaces and the nuclei of endothelial cells (ECs) using lamellipodia and filopodia respectively, to optimize path finding during intraluminal crawling. Well-aligned EC layers or replicas of EC layers, which exhibit topography similar to that of EC layers, were fabricated, and flow was applied either parallel or perpendicular to the orientation of EC alignment. T cells crawled along the valleys of the topographical landscapes of the EC layers, while avoiding nuclei of ECs regardless of flow direction. Pharmacological inhibitor treatments revealed that sensing of topography and nuclei of EC layers was mediated by lamellipodia and filopodia, respectively. Lamellipodia or filopodia-inhibited T cells crawled significantly longer distances for extravasation than did normal T cells, indicating that sensing biophysical cues are critical for optimizing routes for extravasation.

@article{song2014cells, abstract = {Intraluminal crawling is considered to be important for extravasation of leukocytes in blood vessels{,} but biochemical/biophysical cues guiding the crawling of leukocytes have not been clear. Here we provide evidences that T cells sense the topography of luminal surfaces and the nuclei of endothelial cells (ECs) using lamellipodia and filopodia respectively{,} to optimize path finding during intraluminal crawling. Well-aligned EC layers or replicas of EC layers{,} which exhibit topography similar to that of EC layers{,} were fabricated{,} and flow was applied either parallel or perpendicular to the orientation of EC alignment. T cells crawled along the valleys of the topographical landscapes of the EC layers{,} while avoiding nuclei of ECs regardless of flow direction. Pharmacological inhibitor treatments revealed that sensing of topography and nuclei of EC layers was mediated by lamellipodia and filopodia{,} respectively. Lamellipodia or filopodia-inhibited T cells crawled significantly longer distances for extravasation than did normal T cells{,} indicating that sensing biophysical cues are critical for optimizing routes for extravasation.}, added-at = {2014-02-24T11:00:01.000+0100}, author = {Song, Kwang Hoon and Kwon, Keon Woo and Choi, Jong-Cheol and Jung, Jaehwang and Park, YongKeun and Suh, Kahp-Yang and Doh, Junsang}, biburl = {https://www.bibsonomy.org/bibtex/26186fdeda2e6b19ce2d14e57d478d8fd/bkoch}, description = {T cells sense biophysical cues using lamellipodia and filopodia to optimize intraluminal path finding - Integrative Biology (RSC Publishing)}, doi = {10.1039/C4IB00021H}, interhash = {033791887fa35db14b374abe9f7e2425}, intrahash = {6186fdeda2e6b19ce2d14e57d478d8fd}, journal = {Integr. Biol.}, keywords = {filopodia methods migration phd}, pages = {-}, publisher = {The Royal Society of Chemistry}, timestamp = {2014-02-27T13:40:51.000+0100}, title = {T cells sense biophysical cues using lamellipodia and filopodia to optimize intraluminal path finding}, url = {http://dx.doi.org/10.1039/C4IB00021H}, year = 2014 }