%0 Journal Article %1 heene2023promising %A Heene, S. %A Renzelmann, J. %A Müller, C. %A Stanislawski, N. %A Cholewa, F. %A Moosmann, P. %A Blume, H. %A Blume, C. %B Biochemical Engineering Journal %C Biotrics Bioimplants AG, Ullsteinstraße 108, Berlin, D-12109, Germany %D 2023 %I Elsevier B.V. %K Article Bioconversion Bioreactor Bioreactors Cell Cells Clinical Computational Confluent Cultivation Cytology Dynamic Endothelial Endothelial-cells Endothelialization Flow Monolayers Newtonian Non Processes Scaffolds Shear Stress Tissue Vascular acid alignment analysis animal aortic application cadherin cell cells clinical comparative computational contact controlled cultivation dynamics endothelial endothelialization engineered flow fluid glucose graft grafts human image immunofluorescence immunohistochemistry intake lactic laminar mathematical measurement monolayers myown nonhuman parameters physical practice process processing property protocol pulsatile quantitative rate shear simulation stress structure study surface system tissue vascular viscometry %R 10.1016/j.bej.2023.109095 %T A promising protocol for the endothelialization of vascular grafts in an instrumented rotating bioreactor towards clinical application %U https://www.scopus.com/inward/record.uri?eid=2-s2.0-85173455453&doi=10.1016%2fj.bej.2023.109095&partnerID=40&md5=262cfaf5e26b6aefaf4e00182adf3de5 %V 200 %X Pre-endothelialization of a tissue-engineered vascular graft before implantation aims to prevent thrombosis and immunoreactions. This work demonstrates a standardized cultivation process to build a confluent monolayer with human aortal endothelial cells on xenogenous scaffolds. Pre-tested dynamic cultivation conditions in flow slides with pulsatile flow (1 Hz) representing arterial wall conditions were transferred to a newly designed multi-featured rotational bioreactor system. The medium was thickened with 1% methyl cellulose simulating a non-Newtonian fluid comparable to blood. Computational fluid dynamics was used to estimate the optimal volume flow and medium distribution inside the bioreactor chamber for defined wall-near shear stress levels. Flow measurements were performed during cultivation for constant monitoring of the process. Three decellularized porcine arteries were seeded and cultivated in the bioreactor over six days. 1% MC turned out to be the optimal percentage to achieve shear stress values ranging up to 10 dyn/cm2. Vascular endothelial cells formed a continuous monolayer with significant cell alignment in the direction of flow. The presented cultivation protocol in the bioreactor system thus displays a promising template for graft endothelialization and cultivation. Therefore, establishing a key step for future tissue-engineered vascular graft development with a view towards clinical application. © 2023

@article{heene2023promising, abstract = {Pre-endothelialization of a tissue-engineered vascular graft before implantation aims to prevent thrombosis and immunoreactions. This work demonstrates a standardized cultivation process to build a confluent monolayer with human aortal endothelial cells on xenogenous scaffolds. Pre-tested dynamic cultivation conditions in flow slides with pulsatile flow (1 Hz) representing arterial wall conditions were transferred to a newly designed multi-featured rotational bioreactor system. The medium was thickened with 1% methyl cellulose simulating a non-Newtonian fluid comparable to blood. Computational fluid dynamics was used to estimate the optimal volume flow and medium distribution inside the bioreactor chamber for defined wall-near shear stress levels. Flow measurements were performed during cultivation for constant monitoring of the process. Three decellularized porcine arteries were seeded and cultivated in the bioreactor over six days. 1% MC turned out to be the optimal percentage to achieve shear stress values ranging up to 10 dyn/cm2. Vascular endothelial cells formed a continuous monolayer with significant cell alignment in the direction of flow. The presented cultivation protocol in the bioreactor system thus displays a promising template for graft endothelialization and cultivation. Therefore, establishing a key step for future tissue-engineered vascular graft development with a view towards clinical application. © 2023}, added-at = {2024-02-05T16:19:53.000+0100}, address = {Biotrics Bioimplants AG, Ullsteinstraße 108, Berlin, D-12109, Germany}, author = {Heene, S. and Renzelmann, J. and Müller, C. and Stanislawski, N. and Cholewa, F. and Moosmann, P. and Blume, H. and Blume, C.}, biburl = {https://www.bibsonomy.org/bibtex/264ee066055fd8ee744d06797e496ec64/fabcho}, booktitle = {Biochemical Engineering Journal}, comment = {Export Date: 05 February 2024; Cited By: 0; Correspondence Address: C. Blume; Leibniz University Hannover, Institute of Technical Chemistry, Hannover, Callinstr. 5, D-30167, Germany; email: blume@iftc.uni-hannover.de; CODEN: BEJOF}, doi = {10.1016/j.bej.2023.109095}, interhash = {8f47e5516f56b27b5a2c807704ecfab8}, intrahash = {64ee066055fd8ee744d06797e496ec64}, issn = {1369703X}, keywords = {Article Bioconversion Bioreactor Bioreactors Cell Cells Clinical Computational Confluent Cultivation Cytology Dynamic Endothelial Endothelial-cells Endothelialization Flow Monolayers Newtonian Non Processes Scaffolds Shear Stress Tissue Vascular acid alignment analysis animal aortic application cadherin cell cells clinical comparative computational contact controlled cultivation dynamics endothelial endothelialization engineered flow fluid glucose graft grafts human image immunofluorescence immunohistochemistry intake lactic laminar mathematical measurement monolayers myown nonhuman parameters physical practice process processing property protocol pulsatile quantitative rate shear simulation stress structure study surface system tissue vascular viscometry}, publisher = {Elsevier B.V.}, timestamp = {2024-03-05T15:39:50.000+0100}, title = {A promising protocol for the endothelialization of vascular grafts in an instrumented rotating bioreactor towards clinical application}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85173455453&doi=10.1016%2fj.bej.2023.109095&partnerID=40&md5=262cfaf5e26b6aefaf4e00182adf3de5}, volume = 200, year = 2023 }