%0 Journal Article %1 ghaemi2013exploring %A Ghaemi, Soraya Rasi %A Harding, Frances J. %A Delalat, Bahman %A Gronthos, Stan %A Voelcker, Nicolas H. %D 2013 %J Biomaterials %K biomat msc nextgen review screening %N 31 %P 7601--7615 %R http://dx.doi.org/10.1016/j.biomaterials.2013.06.022 %T Exploring the mesenchymal stem cell niche using high throughput screening %U http://www.sciencedirect.com/science/article/pii/S0142961213007084 %V 34 %X Abstract In the field of stem cell technology, future advancements rely on the effective isolation, scale-up and maintenance of specific stem cell populations and robust procedures for their directed differentiation. The stem cell microenvironment – or niche – encompasses signal inputs from stem cells, supporting cells and from the extracellular matrix. In this context, the contribution of physicochemical surface variables is being increasingly recognised. This paradigm can be exploited to exert control over cellular behaviour. However, the number of parameters at play, and their complex interactions, presents a formidable challenge in delineating how the decisions of cell fate are orchestrated within the niche. Additionally, in the case of mesenchymal stem cells (MSC), more than one type of stem cell niche has been identified. By employing high throughput screening (HTS) strategies, common and specific attributes of each \MSC\ niche can be probed. Here, we explore biological, chemical and physical parameters that are known to influence \MSC\ self-renewal and differentiation. We then review techniques and strategies that allow the \HTS\ of surface properties for conditions that direct stem cell fate, using \MSC\ as a case study. Finally, challenges in recapturing the niche, particularly its three dimensional nature, in surface-based \HTS\ formats are discussed.

@article{ghaemi2013exploring, abstract = {Abstract In the field of stem cell technology, future advancements rely on the effective isolation, scale-up and maintenance of specific stem cell populations and robust procedures for their directed differentiation. The stem cell microenvironment – or niche – encompasses signal inputs from stem cells, supporting cells and from the extracellular matrix. In this context, the contribution of physicochemical surface variables is being increasingly recognised. This paradigm can be exploited to exert control over cellular behaviour. However, the number of parameters at play, and their complex interactions, presents a formidable challenge in delineating how the decisions of cell fate are orchestrated within the niche. Additionally, in the case of mesenchymal stem cells (MSC), more than one type of stem cell niche has been identified. By employing high throughput screening (HTS) strategies, common and specific attributes of each \{MSC\} niche can be probed. Here, we explore biological, chemical and physical parameters that are known to influence \{MSC\} self-renewal and differentiation. We then review techniques and strategies that allow the \{HTS\} of surface properties for conditions that direct stem cell fate, using \{MSC\} as a case study. Finally, challenges in recapturing the niche, particularly its three dimensional nature, in surface-based \{HTS\} formats are discussed. }, added-at = {2016-05-23T17:14:20.000+0200}, author = {Ghaemi, Soraya Rasi and Harding, Frances J. and Delalat, Bahman and Gronthos, Stan and Voelcker, Nicolas H.}, biburl = {https://www.bibsonomy.org/bibtex/2703f74b239bf634903da6175284590ae/bkoch}, description = {Exploring the mesenchymal stem cell niche using high throughput screening}, doi = {http://dx.doi.org/10.1016/j.biomaterials.2013.06.022}, interhash = {9a64f9cd2b111281f04270b91e0eb5a0}, intrahash = {703f74b239bf634903da6175284590ae}, issn = {0142-9612}, journal = {Biomaterials }, keywords = {biomat msc nextgen review screening}, number = 31, pages = {7601--7615}, timestamp = {2016-05-23T17:14:20.000+0200}, title = {Exploring the mesenchymal stem cell niche using high throughput screening }, url = {http://www.sciencedirect.com/science/article/pii/S0142961213007084}, volume = 34, year = 2013 }