The apparent translational diffusion coefficients of four 20 base
pair (bp) DNA oligonucleotides with different sequences have been
measured by capillary electrophoresis. using the stopped migration
method The diffusion coefficients of the four oligomers were equal
within experimental error, and averaged (120 +/- 10) x 10(-8) cm(2)
s(-1) in 40 mM Tris-acetate-EDTA buffer at 25 degreesC. Since this
value is nearly identical to the translational diffusion coefficient
determined for a different 20-bp oligomer using other methods, the
stopped migration method can accurately measure the diffusion coefficients
of small DNA oligomers. The apparent diffusion coefficient of a 118-bp
DNA restriction fragment was also measured by the stopped migration
method. However. the observed value was similar to 25% larger than
expected from other measurements, possibly because the diffusion
coefficients of larger DNA molecules are somewhat dependent on the
ionic strength of the solution. (C) 2001 John Wiley & Sons, Inc.
Biopolymers 58: 390-397, 2001.
%0 Journal Article
%1 stellwagen01a
%A Stellwagen, N. C.
%A Magnusdottir, S.
%A Gelfi, C.
%A Righetti, P. G.
%D 2001
%J Biopolymers
%K DISPERSION; DYNAMIC ELECTROPHORESIS; FRAGMENTS; FREE GEL; LIGHT-SCATTERING; MOBILITY; MODEL; PEPTIDES; PROTEINS; SALT SOLUTION ZONE
%N 4
%P 390--397
%T Measuring the translational diffusion coefficients of small DNA molecules
by capillary electrophoresis
%V 58
%X The apparent translational diffusion coefficients of four 20 base
pair (bp) DNA oligonucleotides with different sequences have been
measured by capillary electrophoresis. using the stopped migration
method The diffusion coefficients of the four oligomers were equal
within experimental error, and averaged (120 +/- 10) x 10(-8) cm(2)
s(-1) in 40 mM Tris-acetate-EDTA buffer at 25 degreesC. Since this
value is nearly identical to the translational diffusion coefficient
determined for a different 20-bp oligomer using other methods, the
stopped migration method can accurately measure the diffusion coefficients
of small DNA oligomers. The apparent diffusion coefficient of a 118-bp
DNA restriction fragment was also measured by the stopped migration
method. However. the observed value was similar to 25% larger than
expected from other measurements, possibly because the diffusion
coefficients of larger DNA molecules are somewhat dependent on the
ionic strength of the solution. (C) 2001 John Wiley & Sons, Inc.
Biopolymers 58: 390-397, 2001.
@article{stellwagen01a,
abstract = {The apparent translational diffusion coefficients of four 20 base
pair (bp) DNA oligonucleotides with different sequences have been
measured by capillary electrophoresis. using the stopped migration
method The diffusion coefficients of the four oligomers were equal
within experimental error, and averaged (120 +/- 10) x 10(-8) cm(2)
s(-1) in 40 mM Tris-acetate-EDTA buffer at 25 degreesC. Since this
value is nearly identical to the translational diffusion coefficient
determined for a different 20-bp oligomer using other methods, the
stopped migration method can accurately measure the diffusion coefficients
of small DNA oligomers. The apparent diffusion coefficient of a 118-bp
DNA restriction fragment was also measured by the stopped migration
method. However. the observed value was similar to 25% larger than
expected from other measurements, possibly because the diffusion
coefficients of larger DNA molecules are somewhat dependent on the
ionic strength of the solution. (C) 2001 John Wiley & Sons, Inc.
Biopolymers 58: 390-397, 2001.},
added-at = {2007-06-15T17:33:15.000+0200},
author = {Stellwagen, N. C. and Magnusdottir, S. and Gelfi, C. and Righetti, P. G.},
biburl = {https://www.bibsonomy.org/bibtex/2709c3dfd280810ec232fefd13c170c47/kaigrass},
de = {DNA; translational diffusion coefficients; capillary electrophoresis},
interhash = {d616e1e8fac564736dffbfca3c38526d},
intrahash = {709c3dfd280810ec232fefd13c170c47},
journal = {Biopolymers},
keywords = {DISPERSION; DYNAMIC ELECTROPHORESIS; FRAGMENTS; FREE GEL; LIGHT-SCATTERING; MOBILITY; MODEL; PEPTIDES; PROTEINS; SALT SOLUTION ZONE},
month = {April},
number = 4,
owner = {grass},
pages = {390--397},
sn = {0006-3525},
timestamp = {2007-06-15T17:33:24.000+0200},
title = {Measuring the translational diffusion coefficients of small DNA molecules
by capillary electrophoresis},
ut = {ISI:000167129100004},
volume = 58,
year = 2001
}