Abstract
In this study, we report the antibacterial activity and modulation of
antibiotic activity by Fe-2(MoO4)(3) microstructures obtained by the
hydrothermal route without use of surfactants or organic additives. This
material was characterized by X-ray diffraction (XRD), Raman
spectroscopy and scanning electron microscopy (SEM) images. The XRD
pattern showed that the Fe-2(MoO4)(3) crystallize in a monoclinic
structure without secondary phases. Raman spectroscopy confirms the
formation of Fe-2(MoO4)(3). SEM images show that the Fe-2(MoO4)(3)
obtained have ball-of-yarn shaped morphology. In the antibacterial
assays, strains of Escherichia coil, Pseudomonas aeruginosa and
Staphylococcus aureus were assayed by microdilution method to evaluate
the antibacterial and modulatory-antibiotic activity with antibiotics as
gentamicin, norfloxacin and imipenem. Against all bacteria, the Minimum Inhibitory Concentration (MIC) was Fe-2(MoO4)(3) >= 1024 mu g/mL. This
high MIC result must be associated with the fact of the iron be an
essential microelement to the bacterial growth. However, when the
Fe-2(MoO4)(3) was assayed in association with the antibiotics was
observed an antagonistic effect demonstrated by an enhance of the MIC.
This fact is associated directly with the pro-oxidative properties of
metallic oxides. These compounds enhance the production of free
radicals, as H2O2 and superoxide ions that can affect the cell
structures as cell membrane and cell wall. Other effect is associated
with the possible coordination of the metal, performing bonds with the
chemical structure of the antibiotics, reducing their activity. Our
results indicated that nanocompounds as Fe-2(MoO4)(3) can not be used as
antimicrobial products for clinical usage, neither directly and neither
in association with antibiotics.
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