Design and synthesis of basic functional circuits are the fundamental tasks of synthetic biologists. Before it is possible to engineer higher-order genetic networks that can perform complex functions, a toolkit of basic devices must be developed. Among those devices, sequential logic circuits are expected to be the foundation of the genetic information-processing systems. In this study, we report the design and construction of a genetic sequential logic circuit in Escherichia coli. It can generate different outputs in response to the same input signal on the basis of its internal state, and 'memorize' the output. The circuit is composed of two parts: (1) a bistable switch memory module and (2) a double-repressed promoter NOR gate module. The two modules were individually rationally designed, and they were coupled together by fine-tuning the interconnecting parts through directed evolution. After fine-tuning, the circuit could be repeatedly, alternatively triggered by the same input signal; it functions as a push-on push-off switch.
%0 Journal Article
%1 Lou2010Synthesizing
%A Lou, Chunbo
%A Liu, Xili
%A Ni, Ming
%A Huang, Yiqi
%A Huang, Qiushi
%A Huang, Longwen
%A Jiang, Lingli
%A Lu, Dan
%A Wang, Mingcong
%A Liu, Chang
%A Chen, Daizhuo
%A Chen, Chongyi
%A Chen, Xiaoyue
%A Yang, Le
%A Ma, Haisu
%A Chen, Jianguo
%A Ouyang, Qi
%D 2010
%I Nature Publishing Group
%J Molecular systems biology
%K circuits synthetic-biology
%R 10.1038/msb.2010.2
%T Synthesizing a novel genetic sequential logic circuit: a push-on push-off switch.
%U http://dx.doi.org/10.1038/msb.2010.2
%V 6
%X Design and synthesis of basic functional circuits are the fundamental tasks of synthetic biologists. Before it is possible to engineer higher-order genetic networks that can perform complex functions, a toolkit of basic devices must be developed. Among those devices, sequential logic circuits are expected to be the foundation of the genetic information-processing systems. In this study, we report the design and construction of a genetic sequential logic circuit in Escherichia coli. It can generate different outputs in response to the same input signal on the basis of its internal state, and 'memorize' the output. The circuit is composed of two parts: (1) a bistable switch memory module and (2) a double-repressed promoter NOR gate module. The two modules were individually rationally designed, and they were coupled together by fine-tuning the interconnecting parts through directed evolution. After fine-tuning, the circuit could be repeatedly, alternatively triggered by the same input signal; it functions as a push-on push-off switch.
@article{Lou2010Synthesizing,
abstract = {Design and synthesis of basic functional circuits are the fundamental tasks of synthetic biologists. Before it is possible to engineer higher-order genetic networks that can perform complex functions, a toolkit of basic devices must be developed. Among those devices, sequential logic circuits are expected to be the foundation of the genetic information-processing systems. In this study, we report the design and construction of a genetic sequential logic circuit in Escherichia coli. It can generate different outputs in response to the same input signal on the basis of its internal state, and 'memorize' the output. The circuit is composed of two parts: (1) a bistable switch memory module and (2) a double-repressed promoter {NOR} gate module. The two modules were individually rationally designed, and they were coupled together by fine-tuning the interconnecting parts through directed evolution. After fine-tuning, the circuit could be repeatedly, alternatively triggered by the same input signal; it functions as a push-on push-off switch.},
added-at = {2018-12-02T16:09:07.000+0100},
author = {Lou, Chunbo and Liu, Xili and Ni, Ming and Huang, Yiqi and Huang, Qiushi and Huang, Longwen and Jiang, Lingli and Lu, Dan and Wang, Mingcong and Liu, Chang and Chen, Daizhuo and Chen, Chongyi and Chen, Xiaoyue and Yang, Le and Ma, Haisu and Chen, Jianguo and Ouyang, Qi},
biburl = {https://www.bibsonomy.org/bibtex/273ad64404a2068bc50af218b4bc0cde0/karthikraman},
citeulike-article-id = {6780317},
citeulike-linkout-0 = {http://dx.doi.org/10.1038/msb.2010.2},
citeulike-linkout-1 = {http://dx.doi.org/10.1038/msb20102},
citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/20212522},
citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=20212522},
day = 09,
doi = {10.1038/msb.2010.2},
interhash = {e02a3a17cc5fae12ce8c30209fad4c05},
intrahash = {73ad64404a2068bc50af218b4bc0cde0},
issn = {1744-4292},
journal = {Molecular systems biology},
keywords = {circuits synthetic-biology},
month = mar,
pmid = {20212522},
posted-at = {2010-03-09 12:56:57},
priority = {2},
publisher = {Nature Publishing Group},
timestamp = {2018-12-02T16:09:07.000+0100},
title = {Synthesizing a novel genetic sequential logic circuit: a push-on push-off switch.},
url = {http://dx.doi.org/10.1038/msb.2010.2},
volume = 6,
year = 2010
}