If one accepts that the fundamental pursuit of genetics is to determine
the genotypes that explain phenotypes, the meteoric increase of DNA
sequence information applied toward that pursuit has nowhere to go
but up. The recent introduction of instruments capable of producing
millions of DNA sequence reads in a single run is rapidly changing
the landscape of genetics, providing the ability to answer questions
with heretofore unimaginable speed. These technologies will provide
an inexpensive, genome-wide sequence readout as an endpoint to applications
ranging from chromatin immunoprecipitation, mutation mapping and
polymorphism discovery to noncoding RNA discovery. Here I survey
next-generation sequencing technologies and consider how they can
provide a more complete picture of how the genome shapes the organism.
%0 Journal Article
%1 Mardis08
%A Mardis, Elaine R
%D 2008
%J Trends Genet.
%K Animals Forecasting Genetics,_trends Humans Sequence_Analysis,_DNA
%N 3
%P 133-41
%R 10.1016/j.tig.2007.12.007
%T The impact of next-generation sequencing technology on genetics.
%U http://dx.doi.org/10.1016/j.tig.2007.12.007
%V 24
%X If one accepts that the fundamental pursuit of genetics is to determine
the genotypes that explain phenotypes, the meteoric increase of DNA
sequence information applied toward that pursuit has nowhere to go
but up. The recent introduction of instruments capable of producing
millions of DNA sequence reads in a single run is rapidly changing
the landscape of genetics, providing the ability to answer questions
with heretofore unimaginable speed. These technologies will provide
an inexpensive, genome-wide sequence readout as an endpoint to applications
ranging from chromatin immunoprecipitation, mutation mapping and
polymorphism discovery to noncoding RNA discovery. Here I survey
next-generation sequencing technologies and consider how they can
provide a more complete picture of how the genome shapes the organism.
@article{Mardis08,
abstract = {If one accepts that the fundamental pursuit of genetics is to determine
the genotypes that explain phenotypes, the meteoric increase of DNA
sequence information applied toward that pursuit has nowhere to go
but up. The recent introduction of instruments capable of producing
millions of DNA sequence reads in a single run is rapidly changing
the landscape of genetics, providing the ability to answer questions
with heretofore unimaginable speed. These technologies will provide
an inexpensive, genome-wide sequence readout as an endpoint to applications
ranging from chromatin immunoprecipitation, mutation mapping and
polymorphism discovery to noncoding RNA discovery. Here I survey
next-generation sequencing technologies and consider how they can
provide a more complete picture of how the genome shapes the organism.},
added-at = {2010-01-26T20:35:53.000+0100},
author = {Mardis, Elaine R},
biburl = {https://www.bibsonomy.org/bibtex/27709eefa0874eba85afa4e204a11c0af/denilw},
doi = {10.1016/j.tig.2007.12.007},
file = {article:../ChIP-seq and next-gen sequencing/The impact of next-generation
sequencing technology on genetics.pdf:pdf},
institution = {Genome Sequencing Center, Washington University School of Medicine,
St. Louis, MO 63108, USA. emardis@watson.wustl.edu},
interhash = {6fe4e2ace175d8ec72ea13b42b7e5305},
intrahash = {7709eefa0874eba85afa4e204a11c0af},
journal = {Trends Genet.},
keywords = {Animals Forecasting Genetics,_trends Humans Sequence_Analysis,_DNA},
month = Mar,
number = 3,
owner = {denilw},
pages = {133-41},
pii = {S0168-9525(08)00023-1},
pmid = {18262675},
timestamp = {2010-01-26T20:36:01.000+0100},
title = {The impact of next-generation sequencing technology on genetics.},
url = {http://dx.doi.org/10.1016/j.tig.2007.12.007},
volume = 24,
year = 2008
}