Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) are a major challenge for hospital hygiene. Typing by DNA sequence analysis of the repeat region of the protein A gene (spa) significantly improved typing of MRSA in the hospital setting. However, microevolution of spa repeats in epidemic clones appears to occur at a fairly slow clock rate. Therefore, DNA sequence-based methods providing additional resolution are desirable in some situations. We evaluated the use of the clfB repeat region proposed recently by others as a possible complementation to spa typing. Using epidemic MRSA isolates from two German university hospitals, we show that the clfB repeat region does not offer any additional discriminatory power.
- a,
- adhesins,
- aureus,
- bacterial
- bacterial,
- humans,
- infections,
- methicillin
- methicillin,
- protein
- repeat
- resistance,
- sequences
- staphylococcal
- staphylococcus
- tandem
- techniques,
- typing
- {dna},
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