A sample-scanning confocal optical microscope for single-molecule imaging and spectroscopy working at superfluid helium temperature, liquid nitrogen, and room temperature is described. An optical resolution of 800 nm full width at half maximum as well as a detection efficiency of ≈3.5\% are achieved. The sample scanner features an exceptionally large scan range of 23 μm at 1.8 K. A position sensor allows for continuous observation of the scanner motion and for a correction of piezoelectric hysteresis and creep at 77 K and at room temperature. Coarse positioning of the sample in x-y is achieved by an inertia drive with high reproducibility and nanometer precision. We demonstrate combined high–resolution confocal imaging and spectroscopy of single molecules at 1.8 K.
Segura et al. - 2000 - A sample-scanning confocal optical microscope for .pdf:C\:\\Users\\scherzad\\Zotero\\storage\\3GFG6NEA\\Segura et al. - 2000 - A sample-scanning confocal optical microscope for .pdf:application/pdf;Snapshot:C\:\\Users\\scherzad\\Zotero\\storage\\YJVMM56V\\1.html:text/html
%0 Journal Article
%1 segura2000samplescanning
%A Segura, Jean-Manuel
%A Renn, Alois
%A Hecht, Bert
%D 2000
%J Rev. Sci. Instrum.
%K experiment nano-optics single-molecules spectroscopy
%N 4
%P 1706-1711
%R 10.1063/1.1150524
%T A sample-scanning confocal optical microscope for cryogenic operation
%V 71
%X A sample-scanning confocal optical microscope for single-molecule imaging and spectroscopy working at superfluid helium temperature, liquid nitrogen, and room temperature is described. An optical resolution of 800 nm full width at half maximum as well as a detection efficiency of ≈3.5\% are achieved. The sample scanner features an exceptionally large scan range of 23 μm at 1.8 K. A position sensor allows for continuous observation of the scanner motion and for a correction of piezoelectric hysteresis and creep at 77 K and at room temperature. Coarse positioning of the sample in x-y is achieved by an inertia drive with high reproducibility and nanometer precision. We demonstrate combined high–resolution confocal imaging and spectroscopy of single molecules at 1.8 K.
@article{segura2000samplescanning,
abstract = {A sample-scanning confocal optical microscope for single-molecule imaging and spectroscopy working at superfluid helium temperature, liquid nitrogen, and room temperature is described. An optical resolution of 800 nm full width at half maximum as well as a detection efficiency of ≈3.5\% are achieved. The sample scanner features an exceptionally large scan range of 23 μm at 1.8 K. A position sensor allows for continuous observation of the scanner motion and for a correction of piezoelectric hysteresis and creep at 77 K and at room temperature. Coarse positioning of the sample in x-y is achieved by an inertia drive with high reproducibility and nanometer precision. We demonstrate combined high–resolution confocal imaging and spectroscopy of single molecules at 1.8 K.},
added-at = {2020-02-10T14:38:56.000+0100},
author = {Segura, Jean-Manuel and Renn, Alois and Hecht, Bert},
biburl = {https://www.bibsonomy.org/bibtex/279eb8e62a7762930bcd4f065b686d5d7/ep5optics},
day = 01,
doi = {10.1063/1.1150524},
file = {Segura et al. - 2000 - A sample-scanning confocal optical microscope for .pdf:C\:\\Users\\scherzad\\Zotero\\storage\\3GFG6NEA\\Segura et al. - 2000 - A sample-scanning confocal optical microscope for .pdf:application/pdf;Snapshot:C\:\\Users\\scherzad\\Zotero\\storage\\YJVMM56V\\1.html:text/html},
interhash = {8f4b400ea84da6c284891d75c33ad5f7},
intrahash = {79eb8e62a7762930bcd4f065b686d5d7},
issn = {0034-6748},
journal = {Rev. Sci. Instrum.},
keywords = {experiment nano-optics single-molecules spectroscopy},
month = {04},
number = 4,
pages = {1706-1711},
timestamp = {2020-02-10T14:38:56.000+0100},
title = {A sample-scanning confocal optical microscope for cryogenic operation},
urldate = {2020-02-10},
volume = 71,
year = 2000
}