Microfluidic paper-based chemiluminescence biosensor for simultaneous
determination of glucose and uric acid
Lab On A Chip, 11 (7):
1286--1291 (2011)DOI: 10.1039/c0lc00524j
Abstract
In this study, a novel microfluidic paper-based chemiluminescence
analytical device (mu PCAD) with a simultaneous, rapid, sensitive
and quantitative response for glucose and uric acid was designed.
This novel lab-on-paper biosensor is based on oxidase enzyme reactions
(glucose oxidase and urate oxidase, respectively) and the chemiluminescence
reaction between a rhodanine derivative and generated hydrogen peroxide
in an acid medium. The possible chemiluminescence assay principle
of this mu PCAD is explained. We found that the simultaneous determination
of glucose and uric acid could be achieved by differing the distances
that the glucose and uric acid samples traveled. This lab-on-paper
biosensor could provide reproducible results upon storage at 4 degrees
C for at least 10 weeks. The application test of our mu PCAD was
then successfully performed with the simultaneous determination of
glucose and uric acid in artificial urine. This study shows the successful
integration of the mu PCAD and the chemiluminescence method will
be an easy-to-use, inexpensive, and portable alternative for point-of-care
monitoring.