%0 Journal Article %1 Liu2016Gene %A Liu, Gaowen %A Yong, Mei Y. %A Yurieva, Marina %A Srinivasan, Kandhadayar G. %A Liu, Jaron %A Lim, John S. %A Poidinger, Michael %A Wright, Graham D. %A Zolezzi, Francesca %A Choi, Hyungwon %A Pavelka, Norman %A Rancati, Giulia %D 2016 %I Elsevier %J Cell %K evolvability experimentation gene-essentiality %N 6 %P 1388--1399 %R 10.1016/j.cell.2015.10.069 %T Gene Essentiality Is a Quantitative Property Linked to Cellular Evolvability %U http://dx.doi.org/10.1016/j.cell.2015.10.069 %V 163 %X Gene essentiality is typically determined by assessing the viability of the corresponding mutant cells, but this definition fails to account for the ability of cells to adaptively evolve to genetic perturbations. Here, we performed a stringent screen to assess the degree to which Saccharomyces cerevisiae cells can survive the deletion of ?1,000 individual ?essential? genes and found that ?9\% of these genetic perturbations could in fact be overcome by adaptive evolution. Our analyses uncovered a genome-wide gradient of gene essentiality, with certain essential cellular functions being more ?evolvable? than others. Ploidy changes were prevalent among the evolved mutant strains, and aneuploidy of a specific chromosome was adaptive for a class of evolvable nucleoporin mutants. These data justify a quantitative redefinition of gene essentiality that incorporates both viability and evolvability of the corresponding mutant cells and will enable selection of therapeutic targets associated with lower risk of emergence of drug resistance.

@article{Liu2016Gene, abstract = {Gene essentiality is typically determined by assessing the viability of the corresponding mutant cells, but this definition fails to account for the ability of cells to adaptively evolve to genetic perturbations. Here, we performed a stringent screen to assess the degree to which Saccharomyces cerevisiae cells can survive the deletion of ?1,000 individual ?essential? genes and found that ?9\% of these genetic perturbations could in fact be overcome by adaptive evolution. Our analyses uncovered a genome-wide gradient of gene essentiality, with certain essential cellular functions being more ?evolvable? than others. Ploidy changes were prevalent among the evolved mutant strains, and aneuploidy of a specific chromosome was adaptive for a class of evolvable nucleoporin mutants. These data justify a quantitative redefinition of gene essentiality that incorporates both viability and evolvability of the corresponding mutant cells and will enable selection of therapeutic targets associated with lower risk of emergence of drug resistance.}, added-at = {2018-12-02T16:09:07.000+0100}, author = {Liu, Gaowen and Yong, Mei Y. and Yurieva, Marina and Srinivasan, Kandhadayar G. and Liu, Jaron and Lim, John S. and Poidinger, Michael and Wright, Graham D. and Zolezzi, Francesca and Choi, Hyungwon and Pavelka, Norman and Rancati, Giulia}, biburl = {https://www.bibsonomy.org/bibtex/286e62ce6a5ecd54ce276bfd82e3e4485/karthikraman}, citeulike-article-id = {13847241}, citeulike-linkout-0 = {http://www.cell.com/cell/abstract/S0092-8674(15)01425-7}, citeulike-linkout-1 = {http://dx.doi.org/10.1016/j.cell.2015.10.069}, day = 28, doi = {10.1016/j.cell.2015.10.069}, interhash = {f3a1453174fc590e7dd8a0b6a20819aa}, intrahash = {86e62ce6a5ecd54ce276bfd82e3e4485}, issn = {00928674}, journal = {Cell}, keywords = {evolvability experimentation gene-essentiality}, month = mar, number = 6, pages = {1388--1399}, posted-at = {2016-03-28 08:29:40}, priority = {2}, publisher = {Elsevier}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {Gene Essentiality Is a Quantitative Property Linked to Cellular Evolvability}, url = {http://dx.doi.org/10.1016/j.cell.2015.10.069}, volume = 163, year = 2016 }