Guard cells control the aperture of plant stomata, which are crucial for global fluxes of CO(2) and water. In turn, guard cell anion channels are seen as key players for stomatal closure, but is activation of these channels sufficient to limit plant water loss? To answer this open question, we used an optogenetic approach based on the light-gated anion channelrhodopsin 1 (GtACR1). In tobacco guard cells that express GtACR1, blue- and green-light pulses elicit Cl(-) and NO(3) (-) currents of -1 to -2 nA. The anion currents depolarize the plasma membrane by 60 to 80 mV, which causes opening of voltage-gated K(+) channels and the extrusion of K(+) As a result, continuous stimulation with green light leads to loss of guard cell turgor and closure of stomata at conditions that provoke stomatal opening in wild type. GtACR1 optogenetics thus provides unequivocal evidence that opening of anion channels is sufficient to close stomata.
Huang, Shouguang
Ding, Meiqi
Roelfsema, M Rob G
Dreyer, Ingo
Scherzer, Sonke
Al-Rasheid, Khaled A S
Gao, Shiqiang
Nagel, Georg
Hedrich, Rainer
Konrad, Kai R
eng
2021/07/11
Sci Adv. 2021 Jul 9;7(28):eabg4619. doi: 10.1126/sciadv.abg4619. Print 2021 Jul.
%0 Journal Article
%1 huang2021optogenetic
%A Huang, S.
%A Ding, M.
%A Roelfsema, M. R. G.
%A Dreyer, I.
%A Scherzer, S.
%A Al-Rasheid, K. A. S.
%A Gao, S.
%A Nagel, G.
%A Hedrich, R.
%A Konrad, K. R.
%D 2021
%J Sci Adv
%K imported myOwn uni_network
%N 28
%R 10.1126/sciadv.abg4619
%T Optogenetic control of the guard cell membrane potential and stomatal movement by the light-gated anion channel GtACR1
%U https://www.ncbi.nlm.nih.gov/pubmed/34244145https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270491/pdf/abg4619.pdf
%V 7
%X Guard cells control the aperture of plant stomata, which are crucial for global fluxes of CO(2) and water. In turn, guard cell anion channels are seen as key players for stomatal closure, but is activation of these channels sufficient to limit plant water loss? To answer this open question, we used an optogenetic approach based on the light-gated anion channelrhodopsin 1 (GtACR1). In tobacco guard cells that express GtACR1, blue- and green-light pulses elicit Cl(-) and NO(3) (-) currents of -1 to -2 nA. The anion currents depolarize the plasma membrane by 60 to 80 mV, which causes opening of voltage-gated K(+) channels and the extrusion of K(+) As a result, continuous stimulation with green light leads to loss of guard cell turgor and closure of stomata at conditions that provoke stomatal opening in wild type. GtACR1 optogenetics thus provides unequivocal evidence that opening of anion channels is sufficient to close stomata.
@article{huang2021optogenetic,
abstract = {Guard cells control the aperture of plant stomata, which are crucial for global fluxes of CO(2) and water. In turn, guard cell anion channels are seen as key players for stomatal closure, but is activation of these channels sufficient to limit plant water loss? To answer this open question, we used an optogenetic approach based on the light-gated anion channelrhodopsin 1 (GtACR1). In tobacco guard cells that express GtACR1, blue- and green-light pulses elicit Cl(-) and NO(3) (-) currents of -1 to -2 nA. The anion currents depolarize the plasma membrane by 60 to 80 mV, which causes opening of voltage-gated K(+) channels and the extrusion of K(+) As a result, continuous stimulation with green light leads to loss of guard cell turgor and closure of stomata at conditions that provoke stomatal opening in wild type. GtACR1 optogenetics thus provides unequivocal evidence that opening of anion channels is sufficient to close stomata.},
added-at = {2024-02-15T15:08:22.000+0100},
author = {Huang, S. and Ding, M. and Roelfsema, M. R. G. and Dreyer, I. and Scherzer, S. and Al-Rasheid, K. A. S. and Gao, S. and Nagel, G. and Hedrich, R. and Konrad, K. R.},
biburl = {https://www.bibsonomy.org/bibtex/28b97359b72e45b72c66bb258ad0dfb4c/jvsi_all},
doi = {10.1126/sciadv.abg4619},
interhash = {dd302d0fdfb7ab54bf9dc4675ec6f2c3},
intrahash = {8b97359b72e45b72c66bb258ad0dfb4c},
issn = {2375-2548 (Electronic)
2375-2548 (Linking)},
journal = {Sci Adv},
keywords = {imported myOwn uni_network},
note = {Huang, Shouguang
Ding, Meiqi
Roelfsema, M Rob G
Dreyer, Ingo
Scherzer, Sonke
Al-Rasheid, Khaled A S
Gao, Shiqiang
Nagel, Georg
Hedrich, Rainer
Konrad, Kai R
eng
2021/07/11
Sci Adv. 2021 Jul 9;7(28):eabg4619. doi: 10.1126/sciadv.abg4619. Print 2021 Jul.},
number = 28,
timestamp = {2024-02-15T15:11:55.000+0100},
title = {Optogenetic control of the guard cell membrane potential and stomatal movement by the light-gated anion channel GtACR1},
type = {Journal Article},
url = {https://www.ncbi.nlm.nih.gov/pubmed/34244145https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270491/pdf/abg4619.pdf},
volume = 7,
year = 2021
}