%0 Journal Article %1 ISI:000250580500007 %A Werner, Kay %A Friedt, Wolfgang %A Ordon, Frank %D 2007 %J EUPHYTICA %K BaMMV BaYMV IFZ barley_(Hordeum_vulgare_L) doubled_haploids_(DHs) molecular_markers pyramiding resistance %N 3 %P 323-329 %T Localisation and combination of resistance genes against soil-borne viruses of barley (BaMMV, BaYMV) using doubled haploids and molecular markers %V 158 %X Barley yellow mosaic virus disease caused by different strains of BaYMV and BaMMV is a major threat to winter barley cultivation in Europe. Different resistance genes against these viruses have been mapped and suitable PCR-based markers have been developed. In this respect doubled haploid (DH) populations proved to be advantageous as they facilitate a repeated test for resistance against all agents of the barley yellow mosaic virus complex and besides this, dominant marker systems are as informative as co-dominant ones in DHs due to the lack of heterozygous genotypes. Using DH populations resistance genes rym4, rym5, rym11, rym13, rym15 and the BaYMV/BaYMV-2 resistance of the barley cultivar �Chikurin Ibaraki 1� have been mapped. DHs are also well suited to pyramiding resistance genes against BaMMV and BaYMV. Since homozygous recessive genotypes are more frequent in DHs than in segregating F-2 populations, DHs can be efficiently used to create broad-spectrum resistance and to extend the usability of partly overcome resistance genes. Results from employing two different strategies for pyramiding, based on one and two DH-steps, respectively, combining three recessive resistance genes, i.e. rym4/rym5, rym9 and rym11, are presented. The faster strategy based on one haploidy step resulted in the identification of all three and two-way combinations of the respective resistance genes.

@article{ISI:000250580500007, abstract = {Barley yellow mosaic virus disease caused by different strains of BaYMV and BaMMV is a major threat to winter barley cultivation in Europe. Different resistance genes against these viruses have been mapped and suitable PCR-based markers have been developed. In this respect doubled haploid (DH) populations proved to be advantageous as they facilitate a repeated test for resistance against all agents of the barley yellow mosaic virus complex and besides this, dominant marker systems are as informative as co-dominant ones in DHs due to the lack of heterozygous genotypes. Using DH populations resistance genes rym4, rym5, rym11, rym13, rym15 and the BaYMV/BaYMV-2 resistance of the barley cultivar �Chikurin Ibaraki 1� have been mapped. DHs are also well suited to pyramiding resistance genes against BaMMV and BaYMV. Since homozygous recessive genotypes are more frequent in DHs than in segregating F-2 populations, DHs can be efficiently used to create broad-spectrum resistance and to extend the usability of partly overcome resistance genes. Results from employing two different strategies for pyramiding, based on one and two DH-steps, respectively, combining three recessive resistance genes, i.e. rym4/rym5, rym9 and rym11, are presented. The faster strategy based on one haploidy step resulted in the identification of all three and two-way combinations of the respective resistance genes.}, added-at = {2008-06-04T14:34:51.000+0200}, author = {Werner, Kay and Friedt, Wolfgang and Ordon, Frank}, biburl = {https://www.bibsonomy.org/bibtex/2989387bba9e15d958b257418fad44fa7/ipzgiessen}, interhash = {fd68065e899b416d46f15a22fd01525e}, intrahash = {989387bba9e15d958b257418fad44fa7}, issn = {0014-2336}, journal = {EUPHYTICA}, keywords = {BaMMV BaYMV IFZ barley_(Hordeum_vulgare_L) doubled_haploids_(DHs) molecular_markers pyramiding resistance}, number = 3, pages = {323-329}, timestamp = {2008-07-18T14:09:08.000+0200}, title = {Localisation and combination of resistance genes against soil-borne viruses of barley (BaMMV, BaYMV) using doubled haploids and molecular markers}, volume = 158, year = 2007 }