%0 Journal Article %1 Ottoy1996a %A Ottoy, M. H. %A Varum, K. M. %A Christensen, B. E. %A Anthonsen, M. W. %A Smidsrod, O. %C THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB %D 1996 %I ELSEVIER SCI LTD %J Carbohydr. Polym. %K ;; [ISI:] aqueous-solution; chromatography; dilute distribution; gel-permeation laser-light-scattering; molecular-weight n-acetylation; polymer polysaccharides; %N 4 %P 253 -- 261 %T Preparative and analytical size-exclusion chromatography of chitosans %V 31 %X Two chitosan samples (fraction of acetylated units (F-A) 0.15 and 0.52) were fractionated by preparative size exclusion chromatography (SEC). The molecular weights and molecular weight distributions of the fractions were analyzed by analytical size exclusion chromatography coupled to an on-line low angle laser light scattering detector and a differential refractive index detector (SEC-LALLS-DRI), and their intrinsic viscosities were determined. The exponent (a) of the Mark-Houwink-Kuhn-Sakurada (MHKS) equation was found to be 0.92+/-0.07 and 1.1+/-0.1, respectively, at I=0.1 and pH 4.5. No variation in F-A related to molecular weight was found. Reversible interaction between chitosans and different column packings strongly influenced the log M-V relationships. This interaction was generally most pronounced for the low-F-A chitosan, suggesting that the protonated amino groups are involved. Ammonium acetate buffer reduced this effect and the use of a new type of SEC-packing seemed to eliminate it. The more highly acetylated chitosan also had a more pronounced tendency towards concentration dependent self-association, which most probably involve intermolecular hydrophobic interactions between the acetyl groups. (C) 1997 Elsevier Science Ltd.

@article{Ottoy1996a, __markedentry = {[phpts:6]}, abstract = {Two chitosan samples (fraction of acetylated units (F-A) 0.15 and 0.52) were fractionated by preparative size exclusion chromatography (SEC). The molecular weights and molecular weight distributions of the fractions were analyzed by analytical size exclusion chromatography coupled to an on-line low angle laser light scattering detector and a differential refractive index detector (SEC-LALLS-DRI), and their intrinsic viscosities were determined. The exponent (a) of the Mark-Houwink-Kuhn-Sakurada (MHKS) equation was found to be 0.92+/-0.07 and 1.1+/-0.1, respectively, at I=0.1 and pH 4.5. No variation in F-A related to molecular weight was found. Reversible interaction between chitosans and different column packings strongly influenced the log M-V relationships. This interaction was generally most pronounced for the low-F-A chitosan, suggesting that the protonated amino groups are involved. Ammonium acetate buffer reduced this effect and the use of a new type of SEC-packing seemed to eliminate it. The more highly acetylated chitosan also had a more pronounced tendency towards concentration dependent self-association, which most probably involve intermolecular hydrophobic interactions between the acetyl groups. (C) 1997 Elsevier Science Ltd.}, added-at = {2011-11-04T13:47:04.000+0100}, address = {THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB}, author = {Ottoy, M. H. and Varum, K. M. and Christensen, B. E. and Anthonsen, M. W. and Smidsrod, O.}, biburl = {https://www.bibsonomy.org/bibtex/2a14416246ee22062ed2bb5897539b157/pawelsikorski}, citedref = {AMIJI MM, 1995, CARBOHYD POLYM, V26, P211 ; ANTHONSEN MW, 1993, CARBOHYD POLYM, V22, P193 ; ANTHONSEN MW, 1994, CARBOHYD POLYM, V25, P13 ; ARCIDIACONO S, 1992, BIOTECHNOL BIOENG, V39, P281 ; BERI RG, 1993, CARBOHYD RES, V238, P11 ; BOHDANECKY W, 1982, VISCOSITY POLYM SOLU ; CHRISTENSEN BE, 1993, MACROMOLECULES, V26, P6111 ; CHRISTENSEN BE, 1996, CARBOHYD POLYM, V29, P217 ; DOMARD A, 1984, POLYM COMMUN, V25, P55 ; DOMARD A, 1986, CHITIN NATURE TECHNO, P315 ; DOMARD A, 1989, INT J BIOL MACROMOL, V11, P297 ; DOMARD A, 1991, POLYM COMMUN, V324, P116 ; DRAGET KI, 1992, BIOMATERIALS, V139, P635 ; GOTO A, 1981, P INT SOC CITRICULT, V2, P931 ; GRUBISIC Z, 1967, J POLYM SCI POL LETT, V5, P753 ; HAMIELEC AE, 1978, J LIQ CHROMATOGR, V1, P111 ; HASAGAWA M, 1994, CARBOHYD RES, V262, P161 ; JORDAN RC, 1978, BIOPOLYMERS, V17, P2885 ; KURITA K, 1989, CHEM LETT, P1597 ; LECACHEUX D, 1986, CARBOHYD POLYM, V6, P477 ; MARTIN M, 1982, CHROMATOGRAPHIA, V157, P426 ; MARTINSEN A, 1991, CARBOHYD POLYM, V15, P171 ; OTTOY MH, 1996, CARBOHYD POLYM, V29, P17 ; RINAUDO M, 1993, INT J BIOL MACROMOL, V15, P281 ; SMIDSROD O, 1968, ACTA CHEM SCAND, V22, P797 ; SMIDSROD O, 1984, HYDROBIOLOGIA, V116, P178 ; SUZUKI Y, 1992, DEEP-SEA RES, V39, P185 ; TANFORD C, 1961, PHYSICAL CHEM MACROM ; VARUM KM, 1991, CARBOHYD RES, V211, P17 ; VARUM KM, 1991, FOOD HYDROCOLLOID, V5, P363 ; VARUM KM, 1992, FOOD HYDROCOLLOID, V5, P497 ; VARUM KM, 1994, CARBOHYD POLYM, V25, P65 ; WANG W, 1991, INT J BIOL MACROMOL, V13, P281 ; WU ACM, 1976, J CHROMATOGR, V128, P87 ; YANAKI T, 1983, J APPL POLYM SCI, V28, P873 ; YOMOTA C, 1993, COLLOID POLYM SCI, V271, P76 ; YU LP, 1987, J APPL POLYM SCI, V33, P1909}, interhash = {41ff7cd1e85106e264330d8fdead7044}, intrahash = {a14416246ee22062ed2bb5897539b157}, isifile-dt = {Article}, isifile-ga = {WR531}, isifile-j9 = {CARBOHYD POLYM}, isifile-nr = {37}, isifile-pi = {OXFORD}, isifile-rp = {Ottoy, MH, NORWEGIAN UNIV SCI & TECHNOL,NORWEGIAN BIOPOLYMER LAB,DEPT ; BIOTECHNOL,N-7034 TRONDHEIM,NORWAY.}, isifile-sc = {Chemistry, Applied; Chemistry, Organic; Polymer Science}, isifile-tc = {31}, issn = {0144-8617}, journal = {Carbohydr. Polym.}, keywords = {;; [ISI:] aqueous-solution; chromatography; dilute distribution; gel-permeation laser-light-scattering; molecular-weight n-acetylation; polymer polysaccharides;}, language = {English}, month = DEC, number = 4, owner = {phpts}, pages = {253 -- 261}, publisher = {ELSEVIER SCI LTD}, size = {9 p.}, sourceid = {ISI:A1996WR53100009}, timestamp = {2011-11-04T13:47:21.000+0100}, title = {Preparative and analytical size-exclusion chromatography of chitosans}, volume = 31, year = 1996 }