%0 Journal Article %1 citeulike:489549 %A Calvo, C. %A Bustos, P. %A Giraudo, P. %A Ulloa, N. %A Sepulveda, J. %A Verdugo, C. %A Toledo, L. %C Departamento de Bioquímica Clinicae Immunología, Facultad de Farmacia, Universidad de Concepción, Chile. %D 1997 %J Journal of Atherosclerosis and Thrombosis %K elisa apob %N 1 %P 45--49 %T A monoclonal antibody-based enzyme immunoassay for the measurement of native and glycated apolipoprotein B-containing particles. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9583354 %V 4 %X We describe the development of sandwich enzyme-linked immunosorbent assays designed to measure native and glycated apolipoprotein B containing particles in plasma. The assays utilize monoclonal antibodies anti native or glycated apo B-LDL for coating and a polyclonal anti apoB-LDL-peroxidase conjugate as the detecting antibody. The method is specific, sensitive and precise. The intra assay coefficient of variation for the plasma native and glycated apolipoprotein B-containing particles was determine to be 7.8% and 7.5%, respectively. The method described can provide specific and reproducible determinations of apoB and glycated-apoB containing particles in plasma; it will be of great interest in the evaluation of atherosclerotic risk in dyslipoproteinemic states in diabetic and non-diabetic subjects.

@article{citeulike:489549, abstract = {We describe the development of sandwich enzyme-linked immunosorbent assays designed to measure native and glycated apolipoprotein B containing particles in plasma. The assays utilize monoclonal antibodies anti native or glycated apo B-LDL for coating and a polyclonal anti apoB-LDL-peroxidase conjugate as the detecting antibody. The method is specific, sensitive and precise. The intra assay coefficient of variation for the plasma native and glycated apolipoprotein B-containing particles was determine to be 7.8% and 7.5%, respectively. The method described can provide specific and reproducible determinations of apoB and glycated-apoB containing particles in plasma; it will be of great interest in the evaluation of atherosclerotic risk in dyslipoproteinemic states in diabetic and non-diabetic subjects.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Departamento de Bioquímica Clinicae Immunología, Facultad de Farmacia, Universidad de Concepción, Chile.}, author = {Calvo, C. and Bustos, P. and Giraudo, P. and Ulloa, N. and Sepulveda, J. and Verdugo, C. and Toledo, L.}, biburl = {https://www.bibsonomy.org/bibtex/2b92b0f54a9fcfcb8bb39eee1db9cc303/biblio24}, citeulike-article-id = {489549}, interhash = {2a41f12d24e30daceb9790365cf141bd}, intrahash = {b92b0f54a9fcfcb8bb39eee1db9cc303}, issn = {1340-3478}, journal = {Journal of Atherosclerosis and Thrombosis}, keywords = {elisa apob}, number = 1, pages = {45--49}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {A monoclonal antibody-based enzyme immunoassay for the measurement of native and glycated apolipoprotein B-containing particles.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=9583354}, volume = 4, year = 1997 }