Introduction: Inflammation due to diabetes mellitus has been understudied. Gongronema latifolium (GL) is reported to have anti-inflammatory potentials. This work was designed to evaluate the effect of ethanolic leaf extract of Gongronema latifolium on some inflammatory markers in Streptozotocin (STZ)-induced diabetic rats.
Materials and methods: 40 Wistar rats of both sexes (150 g-200 g) were divided into 5 groups of eight (8) rats each. Group 1; control received normal saline placebo orally. Group 2; GL only received 200 mg/kg of GL orally. Group 3 DM only received 65 mg/kg bw of STZ intraperitoneally for two days with an interval of one day in between. Group 4; DM + GL received 65 mg/kg bw of STZ + 200mg/kg bw of GL. Group 5; DM + Insulin received 65mg/kgbw of STZ followed by 10 IU/kgbw of insulin subcutaneously. For further inflammatory study, paw oedema was induced by sub-plantar injection of 2% formalin in two groups before and after administration of 200 mg/kg bw of GL daily for 7 days. Blood samples were collected via cardiac puncture for biochemical analysis.
Results: Results from this study showed a significant (p<0.05) increase in blood glucose DM group, compared to the control. Intervention with GL and insulin significantly reduced the glucose level towards normal. Interleukin-6 concentration and C-reactive proteins were significantly (p<0.01) higher in DM group compared with the GL and control groups. Paw oedema size was significantly (p<0.05) increased in the formalin treated group compared with the control. Pretreatment and post treatment with GL significantly (p<0.05) reduced the paw oedema size.
Conclusion: In conclusion, GL (like insulin) has hypoglycemic and anti-inflammatory potentials.
%0 Journal Article
%1 gabriel_otu_ujong_2022_6966795
%A Ujong, Gabriel Otu
%A Beshel, Justin Atiang
%A Okon, Idara Asuquo
%A Owu, Daniel Udofia
%D 2022
%J World Journal of Biology Pharmacy and Health Sciences
%K Gongronema latifolium
%N 1
%P 077–084
%R 10.30574/wjbphs.2022.11.1.0109
%T Anti-inflammatory potentials of ethanolic leaf extract of Gongronema latifolium in streptozotocin induced diabetic rats
%U https://wjbphs.com/content/anti-inflammatory-potentials-ethanolic-leaf-extract-gongronema-latifolium-streptozotocin
%V 11
%X Introduction: Inflammation due to diabetes mellitus has been understudied. Gongronema latifolium (GL) is reported to have anti-inflammatory potentials. This work was designed to evaluate the effect of ethanolic leaf extract of Gongronema latifolium on some inflammatory markers in Streptozotocin (STZ)-induced diabetic rats.
Materials and methods: 40 Wistar rats of both sexes (150 g-200 g) were divided into 5 groups of eight (8) rats each. Group 1; control received normal saline placebo orally. Group 2; GL only received 200 mg/kg of GL orally. Group 3 DM only received 65 mg/kg bw of STZ intraperitoneally for two days with an interval of one day in between. Group 4; DM + GL received 65 mg/kg bw of STZ + 200mg/kg bw of GL. Group 5; DM + Insulin received 65mg/kgbw of STZ followed by 10 IU/kgbw of insulin subcutaneously. For further inflammatory study, paw oedema was induced by sub-plantar injection of 2% formalin in two groups before and after administration of 200 mg/kg bw of GL daily for 7 days. Blood samples were collected via cardiac puncture for biochemical analysis.
Results: Results from this study showed a significant (p<0.05) increase in blood glucose DM group, compared to the control. Intervention with GL and insulin significantly reduced the glucose level towards normal. Interleukin-6 concentration and C-reactive proteins were significantly (p<0.01) higher in DM group compared with the GL and control groups. Paw oedema size was significantly (p<0.05) increased in the formalin treated group compared with the control. Pretreatment and post treatment with GL significantly (p<0.05) reduced the paw oedema size.
Conclusion: In conclusion, GL (like insulin) has hypoglycemic and anti-inflammatory potentials.
@article{gabriel_otu_ujong_2022_6966795,
abstract = {Introduction: Inflammation due to diabetes mellitus has been understudied. Gongronema latifolium (GL) is reported to have anti-inflammatory potentials. This work was designed to evaluate the effect of ethanolic leaf extract of Gongronema latifolium on some inflammatory markers in Streptozotocin (STZ)-induced diabetic rats.
Materials and methods: 40 Wistar rats of both sexes (150 g-200 g) were divided into 5 groups of eight (8) rats each. Group 1; control received normal saline placebo orally. Group 2; GL only received 200 mg/kg of GL orally. Group 3 DM only received 65 mg/kg bw of STZ intraperitoneally for two days with an interval of one day in between. Group 4; DM + GL received 65 mg/kg bw of STZ + 200mg/kg bw of GL. Group 5; DM + Insulin received 65mg/kgbw of STZ followed by 10 IU/kgbw of insulin subcutaneously. For further inflammatory study, paw oedema was induced by sub-plantar injection of 2% formalin in two groups before and after administration of 200 mg/kg bw of GL daily for 7 days. Blood samples were collected via cardiac puncture for biochemical analysis.
Results: Results from this study showed a significant (p<0.05) increase in blood glucose DM group, compared to the control. Intervention with GL and insulin significantly reduced the glucose level towards normal. Interleukin-6 concentration and C-reactive proteins were significantly (p<0.01) higher in DM group compared with the GL and control groups. Paw oedema size was significantly (p<0.05) increased in the formalin treated group compared with the control. Pretreatment and post treatment with GL significantly (p<0.05) reduced the paw oedema size.
Conclusion: In conclusion, GL (like insulin) has hypoglycemic and anti-inflammatory potentials. },
added-at = {2022-11-14T15:16:30.000+0100},
author = {Ujong, Gabriel Otu and Beshel, Justin Atiang and Okon, Idara Asuquo and Owu, Daniel Udofia},
biburl = {https://www.bibsonomy.org/bibtex/2a82d853178f02250af7e627952b3e1be/wjbphsjournal},
doi = {10.30574/wjbphs.2022.11.1.0109},
interhash = {195dd732e8378566d3036de61f5a92e8},
intrahash = {a82d853178f02250af7e627952b3e1be},
issn = {2582-5542},
journal = {{World Journal of Biology Pharmacy and Health Sciences}},
keywords = {Gongronema latifolium},
month = jul,
number = 1,
pages = {077–084},
timestamp = {2022-11-14T15:16:30.000+0100},
title = {Anti-inflammatory potentials of ethanolic leaf extract of Gongronema latifolium in streptozotocin induced diabetic rats},
url = {https://wjbphs.com/content/anti-inflammatory-potentials-ethanolic-leaf-extract-gongronema-latifolium-streptozotocin},
volume = 11,
year = 2022
}