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Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes

, , , , , , , and . ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 47 (33): 6172-6176 (2008)

Abstract

Eagle eyes: dSTORM uses conventional photoswitchable fluorescent dyes that can be reversibly cycled between a fluorescent and a dark state by irradiation with light of different wavelengths (see picture). This elegant approach can visualize cellular structures with a resolution of approximately 20 nm, far beyond the diffraction limit of light, without the need of an activator molecule.

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