General Equation for Peak Capacity in Column Chromatography
Analytical Chemistry, 70 (18):
3853–3856 (1998)DOI: 10.1021/ac9802426
Abstract
In this study, the peak capacities for packed capillaries used in various forms of chromatography were investigated. Experiments found that the peak width at half-height (w1/2) linearly increased with increasing retention time when operating under isothermal, isobaric, and isocratic conditions in capillary gas chromatography, solvating gas chromatography, supercritical fluid chromatography, and liquid chromatography. From this relationship, a general peak capacity (n) expression was obtained. This expression covers conventional packed, packed capillary, and open tubular column chromatographies. With this general expression, it is possible to directly compare the separating capabilities of the various column types and chromatographic techniques.