A spectrophotometric method for the determination of N-nitroso compounds by flow injection analysis, based on a denitrosation reaction with hydrogen bromide - acetic acid followed by reaction of the liberated nitrite with the Griess reagent, is described. Under the optimum reaction conditions, the detection limit for N-nitrosopyrrolidine (NPYR) was 5 x 10^-8 M for a sample volume of 200 pl and the relative standard deviations (n = 9) for 1 x 10^-7 and 5.4 x 10^-6 M NPYR were 3.8 and 0.5%, respectively. Various other N-nitroso compounds gave a similar level of response. Phenol, sorbic acid and methanol did not interfere with the determinations, whereas piperazine interfered seriously with the determination of nitrosoproline. The sampling rate was 25 samples h-1. The method was successfully applied to the analysis of two anti-cancer drug formulations, viz., nimustine hydrochloride and l-(2-chloroethyl)-3-(methyl-alpha-D-glucopyranos-6-yl)-1-nitrosourea injections.
%0 Journal Article
%1 Ohta1988
%A Ohta, Takafumi
%A Goto, Nozomi
%A Takitani, Shoji
%D 1988
%J Analyst
%K absorbance flow-injection hardware nitroso
%P 1333
%R 10.1039/AN9881301333
%T Spectrophotometric Determination of n-Nitroso Compounds by Flow Injection Analysis
%V 113
%X A spectrophotometric method for the determination of N-nitroso compounds by flow injection analysis, based on a denitrosation reaction with hydrogen bromide - acetic acid followed by reaction of the liberated nitrite with the Griess reagent, is described. Under the optimum reaction conditions, the detection limit for N-nitrosopyrrolidine (NPYR) was 5 x 10^-8 M for a sample volume of 200 pl and the relative standard deviations (n = 9) for 1 x 10^-7 and 5.4 x 10^-6 M NPYR were 3.8 and 0.5%, respectively. Various other N-nitroso compounds gave a similar level of response. Phenol, sorbic acid and methanol did not interfere with the determinations, whereas piperazine interfered seriously with the determination of nitrosoproline. The sampling rate was 25 samples h-1. The method was successfully applied to the analysis of two anti-cancer drug formulations, viz., nimustine hydrochloride and l-(2-chloroethyl)-3-(methyl-alpha-D-glucopyranos-6-yl)-1-nitrosourea injections.
@article{Ohta1988,
abstract = {A spectrophotometric method for the determination of N-nitroso compounds by flow injection analysis, based on a denitrosation reaction with hydrogen bromide - acetic acid followed by reaction of the liberated nitrite with the Griess reagent, is described. Under the optimum reaction conditions, the detection limit for N-nitrosopyrrolidine (NPYR) was 5 x 10^-8 M for a sample volume of 200 pl and the relative standard deviations (n = 9) for 1 x 10^-7 and 5.4 x 10^-6 M NPYR were 3.8 and 0.5%, respectively. Various other N-nitroso compounds gave a similar level of response. Phenol, sorbic acid and methanol did not interfere with the determinations, whereas piperazine interfered seriously with the determination of nitrosoproline. The sampling rate was 25 samples h-1. The method was successfully applied to the analysis of two anti-cancer drug formulations, viz., nimustine hydrochloride and l-(2-chloroethyl)-3-(methyl-alpha-D-glucopyranos-6-yl)-1-nitrosourea injections.},
added-at = {2010-03-09T21:44:07.000+0100},
author = {Ohta, Takafumi and Goto, Nozomi and Takitani, Shoji},
biburl = {https://www.bibsonomy.org/bibtex/2bebeb1d86990d272ef0dabba370f95d3/afcallender},
doi = {10.1039/AN9881301333},
file = {Ohta1988.pdf:indexed\\Ohta1988.pdf:PDF},
interhash = {fd6c765a6084e53229f3f9c854c87365},
intrahash = {bebeb1d86990d272ef0dabba370f95d3},
journal = {Analyst},
keywords = {absorbance flow-injection hardware nitroso},
note = {teaching},
pages = 1333,
timestamp = {2010-03-09T22:21:42.000+0100},
title = {Spectrophotometric Determination of n-Nitroso Compounds by Flow Injection Analysis},
volume = 113,
year = 1988
}