Widespread receptivity to neuropeptide PDF throughout the neuronal
circadian clock network of Drosophila revealed by real-time cyclic
AMP imaging
O. Shafer, D. Kim, R. Dunbar-Yaffe, V. Nikolaev, M. Lohse, und P. Taghert. Neuron, 58 (2):
223-37(April 2008)Shafer, Orie T Kim, Dong Jo Dunbar-Yaffe, Richard Nikolaev, Viacheslav
O Lohse, Martin J Taghert, Paul H 2F32NS053222-02/NS/NINDS NIH HHS/United
States MH067122/MH/NIMH NIH HHS/United States NS057105/NS/NINDS NIH
HHS/United States NS21749/NS/NINDS NIH HHS/United States P30 NS057105-039005/NS/NINDS
NIH HHS/United States R01 MH067122-04/MH/NIMH NIH HHS/United States
R01 MH067122-05/MH/NIMH NIH HHS/United States R01 NS021749-21/NS/NINDS
NIH HHS/United States Research Support, N.I.H., Extramural United
States Neuron Neuron. 2008 Apr 24;58(2):223-37..
Zusammenfassung
The neuropeptide PDF is released by sixteen clock neurons in Drosophila
and helps maintain circadian activity rhythms by coordinating a network
of approximately 150 neuronal clocks. Whether PDF acts directly on
elements of this neural network remains unknown. We address this
question by adapting Epac1-camps, a genetically encoded cAMP FRET
sensor, for use in the living brain. We find that a subset of the
PDF-expressing neurons respond to PDF with long-lasting cAMP increases
and confirm that such responses require the PDF receptor. In contrast,
an unrelated Drosophila neuropeptide, DH31, stimulates large cAMP
increases in all PDF-expressing clock neurons. Thus, the network
of approximately 150 clock neurons displays widespread, though not
uniform, PDF receptivity. This work introduces a sensitive means
of measuring cAMP changes in a living brain with subcellular resolution.
Specifically, it experimentally confirms the longstanding hypothesis
that PDF is a direct modulator of most neurons in the Drosophila
clock network.
Shafer, Orie T Kim, Dong Jo Dunbar-Yaffe, Richard Nikolaev, Viacheslav
O Lohse, Martin J Taghert, Paul H 2F32NS053222-02/NS/NINDS NIH HHS/United
States MH067122/MH/NIMH NIH HHS/United States NS057105/NS/NINDS NIH
HHS/United States NS21749/NS/NINDS NIH HHS/United States P30 NS057105-039005/NS/NINDS
NIH HHS/United States R01 MH067122-04/MH/NIMH NIH HHS/United States
R01 MH067122-05/MH/NIMH NIH HHS/United States R01 NS021749-21/NS/NINDS
NIH HHS/United States Research Support, N.I.H., Extramural United
States Neuron Neuron. 2008 Apr 24;58(2):223-37.
%0 Journal Article
%1 Shafer2008
%A Shafer, O. T.
%A Kim, D. J.
%A Dunbar-Yaffe, R.
%A Nikolaev, V. O.
%A Lohse, M. J.
%A Taghert, P. H.
%D 2008
%J Neuron
%K AMP/*metabolism Activity/genetics Animal Animals Animals, Behavior, Brain/cytology Circadian Computer Cyclic Diagnostic Dose-Response Drosophila Drug Expression Forskolin/pharmacology Gene Genetically Hormones/genetics/metabolism Imaging Insect Luminescent Modified Motor Neurons/drug Neuropeptides/*metabolism/pharmacology Proteins/genetics/*metabolism/pharmacology Proteins/metabolism Regulation/drug Relationship, Rhythm/*physiology Systems effects/*physiology
%N 2
%P 223-37
%T Widespread receptivity to neuropeptide PDF throughout the neuronal
circadian clock network of Drosophila revealed by real-time cyclic
AMP imaging
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18439407
%V 58
%X The neuropeptide PDF is released by sixteen clock neurons in Drosophila
and helps maintain circadian activity rhythms by coordinating a network
of approximately 150 neuronal clocks. Whether PDF acts directly on
elements of this neural network remains unknown. We address this
question by adapting Epac1-camps, a genetically encoded cAMP FRET
sensor, for use in the living brain. We find that a subset of the
PDF-expressing neurons respond to PDF with long-lasting cAMP increases
and confirm that such responses require the PDF receptor. In contrast,
an unrelated Drosophila neuropeptide, DH31, stimulates large cAMP
increases in all PDF-expressing clock neurons. Thus, the network
of approximately 150 clock neurons displays widespread, though not
uniform, PDF receptivity. This work introduces a sensitive means
of measuring cAMP changes in a living brain with subcellular resolution.
Specifically, it experimentally confirms the longstanding hypothesis
that PDF is a direct modulator of most neurons in the Drosophila
clock network.
@article{Shafer2008,
abstract = {The neuropeptide PDF is released by sixteen clock neurons in Drosophila
and helps maintain circadian activity rhythms by coordinating a network
of approximately 150 neuronal clocks. Whether PDF acts directly on
elements of this neural network remains unknown. We address this
question by adapting Epac1-camps, a genetically encoded cAMP FRET
sensor, for use in the living brain. We find that a subset of the
PDF-expressing neurons respond to PDF with long-lasting cAMP increases
and confirm that such responses require the PDF receptor. In contrast,
an unrelated Drosophila neuropeptide, DH31, stimulates large cAMP
increases in all PDF-expressing clock neurons. Thus, the network
of approximately 150 clock neurons displays widespread, though not
uniform, PDF receptivity. This work introduces a sensitive means
of measuring cAMP changes in a living brain with subcellular resolution.
Specifically, it experimentally confirms the longstanding hypothesis
that PDF is a direct modulator of most neurons in the Drosophila
clock network.},
added-at = {2010-12-14T18:12:02.000+0100},
author = {Shafer, O. T. and Kim, D. J. and Dunbar-Yaffe, R. and Nikolaev, V. O. and Lohse, M. J. and Taghert, P. H.},
biburl = {https://www.bibsonomy.org/bibtex/2c2dfb2a721f80478ae554c07cb62952e/pharmawuerz},
endnotereftype = {Journal Article},
interhash = {8dd4b5ad8999f005f91aa8bb59358d6a},
intrahash = {c2dfb2a721f80478ae554c07cb62952e},
issn = {1097-4199 (Electronic) 1097-4199 (Linking)},
journal = {Neuron},
keywords = {AMP/*metabolism Activity/genetics Animal Animals Animals, Behavior, Brain/cytology Circadian Computer Cyclic Diagnostic Dose-Response Drosophila Drug Expression Forskolin/pharmacology Gene Genetically Hormones/genetics/metabolism Imaging Insect Luminescent Modified Motor Neurons/drug Neuropeptides/*metabolism/pharmacology Proteins/genetics/*metabolism/pharmacology Proteins/metabolism Regulation/drug Relationship, Rhythm/*physiology Systems effects/*physiology},
month = {Apr 24},
note = {Shafer, Orie T Kim, Dong Jo Dunbar-Yaffe, Richard Nikolaev, Viacheslav
O Lohse, Martin J Taghert, Paul H 2F32NS053222-02/NS/NINDS NIH HHS/United
States MH067122/MH/NIMH NIH HHS/United States NS057105/NS/NINDS NIH
HHS/United States NS21749/NS/NINDS NIH HHS/United States P30 NS057105-039005/NS/NINDS
NIH HHS/United States R01 MH067122-04/MH/NIMH NIH HHS/United States
R01 MH067122-05/MH/NIMH NIH HHS/United States R01 NS021749-21/NS/NINDS
NIH HHS/United States Research Support, N.I.H., Extramural United
States Neuron Neuron. 2008 Apr 24;58(2):223-37.},
number = 2,
pages = {223-37},
shorttitle = {Widespread receptivity to neuropeptide PDF throughout the neuronal
circadian clock network of Drosophila revealed by real-time cyclic
AMP imaging},
timestamp = {2010-12-14T18:12:32.000+0100},
title = {Widespread receptivity to neuropeptide PDF throughout the neuronal
circadian clock network of Drosophila revealed by real-time cyclic
AMP imaging},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18439407},
volume = 58,
year = 2008
}