Kinetics of G-protein-coupled receptor signalling and desensitization
C. Krasel, J. Vilardaga, M. Bunemann, and M. Lohse. Biochem Soc Trans, 32 (Pt 6):
1029-31(December 2004)Krasel, C Vilardaga, J-P Bunemann, M Lohse, M J Research Support,
Non-U.S. Gov't England Biochemical Society transactions Biochem Soc
Trans. 2004 Dec;32(Pt 6):1029-31..
Abstract
The kinetics of G-protein-coupled receptor activation and deactivation
has, so far, been measured only indirectly, most frequently by assessing
the production of various second messengers. We have developed methods
based on fluorescence resonance energy transfer to quantify the kinetics
of receptor activation by agonist (measured as conformational change
in the receptor), the kinetics of G-protein activation (measured
as G-protein subunit rearrangement) and the kinetics of receptor
inactivation by arrestins (measured as receptor-arrestin interaction).
Using these methods, we show that receptor activation by agonists
and signalling to G-proteins occur on the subsecond time scale, whereas
receptor desensitization is limited by receptor phosphorylation and
proceeds more slowly.
Krasel, C Vilardaga, J-P Bunemann, M Lohse, M J Research Support,
Non-U.S. Gov't England Biochemical Society transactions Biochem Soc
Trans. 2004 Dec;32(Pt 6):1029-31.
%0 Journal Article
%1 Krasel2004
%A Krasel, C.
%A Vilardaga, J. P.
%A Bunemann, M.
%A Lohse, M. J.
%D 2004
%J Biochem Soc Trans
%K AMP-Dependent Animals Cyclic Energy Fluorescence G-Protein-Coupled/*physiology Humans Kinases Kinases/metabolism Kinetics Protein Receptor Resonance Signal Transduction/*physiology Transfer beta-Adrenergic
%N Pt 6
%P 1029-31
%T Kinetics of G-protein-coupled receptor signalling and desensitization
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15506955
%V 32
%X The kinetics of G-protein-coupled receptor activation and deactivation
has, so far, been measured only indirectly, most frequently by assessing
the production of various second messengers. We have developed methods
based on fluorescence resonance energy transfer to quantify the kinetics
of receptor activation by agonist (measured as conformational change
in the receptor), the kinetics of G-protein activation (measured
as G-protein subunit rearrangement) and the kinetics of receptor
inactivation by arrestins (measured as receptor-arrestin interaction).
Using these methods, we show that receptor activation by agonists
and signalling to G-proteins occur on the subsecond time scale, whereas
receptor desensitization is limited by receptor phosphorylation and
proceeds more slowly.
@article{Krasel2004,
abstract = {The kinetics of G-protein-coupled receptor activation and deactivation
has, so far, been measured only indirectly, most frequently by assessing
the production of various second messengers. We have developed methods
based on fluorescence resonance energy transfer to quantify the kinetics
of receptor activation by agonist (measured as conformational change
in the receptor), the kinetics of G-protein activation (measured
as G-protein subunit rearrangement) and the kinetics of receptor
inactivation by arrestins (measured as receptor-arrestin interaction).
Using these methods, we show that receptor activation by agonists
and signalling to G-proteins occur on the subsecond time scale, whereas
receptor desensitization is limited by receptor phosphorylation and
proceeds more slowly.},
added-at = {2010-12-14T18:12:02.000+0100},
author = {Krasel, C. and Vilardaga, J. P. and Bunemann, M. and Lohse, M. J.},
biburl = {https://www.bibsonomy.org/bibtex/2c44218a4a1e3e667cd5901d557491307/pharmawuerz},
endnotereftype = {Journal Article},
interhash = {34efed1580904d6a0a8e96bee2ca4080},
intrahash = {c44218a4a1e3e667cd5901d557491307},
issn = {0300-5127 (Print) 0300-5127 (Linking)},
journal = {Biochem Soc Trans},
keywords = {AMP-Dependent Animals Cyclic Energy Fluorescence G-Protein-Coupled/*physiology Humans Kinases Kinases/metabolism Kinetics Protein Receptor Resonance Signal Transduction/*physiology Transfer beta-Adrenergic},
month = Dec,
note = {Krasel, C Vilardaga, J-P Bunemann, M Lohse, M J Research Support,
Non-U.S. Gov't England Biochemical Society transactions Biochem Soc
Trans. 2004 Dec;32(Pt 6):1029-31.},
number = {Pt 6},
pages = {1029-31},
shorttitle = {Kinetics of G-protein-coupled receptor signalling and desensitization},
timestamp = {2010-12-14T18:20:05.000+0100},
title = {Kinetics of G-protein-coupled receptor signalling and desensitization},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15506955},
volume = 32,
year = 2004
}