The complex gene expression responses of Anopheles gambiae to microbial and malaria challenges, injury, and oxidative stress (in the mosquito and/or a cultured cell line) were surveyed by using cDNA microarrays constructed from an EST-clone collection. The expression profiles were broadly subdivided into induced and down-regulated gene clusters. Gram+ and Gram- bacteria and microbial elicitors up-regulated a diverse set of genes, many belonging to the immunity class, and the response to malaria partially overlapped with this response. Oxidative stress activated a distinctive set of genes, mainly implicated in oxidoreductive processes. Injury up- and down-regulated gene clusters also were distinctive, prominently implicating glycolysis-related genes and citric acid cycle/oxidative phosphorylation/redox-mitochondrial functions, respectively. Cross-comparison of in vivo and in vitro responses indicated the existence of tightly coregulated gene groups that may correspond to gene pathways.
%0 Journal Article
%1 pmid12077297
%A Dimopoulos, G.
%A Christophides, G. K.
%A Meister, S.
%A Schultz, J.
%A White, K. P.
%A Barillas-Mury, C.
%A Kafatos, F. C.
%D 2002
%J Proc. Natl. Acad. Sci. U.S.A.
%K imported
%P 8814--8819
%T Genome expression analysis of Anopheles gambiae: responses to injury, bacterial challenge, and malaria infection
%V 99
%X The complex gene expression responses of Anopheles gambiae to microbial and malaria challenges, injury, and oxidative stress (in the mosquito and/or a cultured cell line) were surveyed by using cDNA microarrays constructed from an EST-clone collection. The expression profiles were broadly subdivided into induced and down-regulated gene clusters. Gram+ and Gram- bacteria and microbial elicitors up-regulated a diverse set of genes, many belonging to the immunity class, and the response to malaria partially overlapped with this response. Oxidative stress activated a distinctive set of genes, mainly implicated in oxidoreductive processes. Injury up- and down-regulated gene clusters also were distinctive, prominently implicating glycolysis-related genes and citric acid cycle/oxidative phosphorylation/redox-mitochondrial functions, respectively. Cross-comparison of in vivo and in vitro responses indicated the existence of tightly coregulated gene groups that may correspond to gene pathways.
@article{pmid12077297,
abstract = {The complex gene expression responses of Anopheles gambiae to microbial and malaria challenges, injury, and oxidative stress (in the mosquito and/or a cultured cell line) were surveyed by using cDNA microarrays constructed from an EST-clone collection. The expression profiles were broadly subdivided into induced and down-regulated gene clusters. Gram+ and Gram- bacteria and microbial elicitors up-regulated a diverse set of genes, many belonging to the immunity class, and the response to malaria partially overlapped with this response. Oxidative stress activated a distinctive set of genes, mainly implicated in oxidoreductive processes. Injury up- and down-regulated gene clusters also were distinctive, prominently implicating glycolysis-related genes and citric acid cycle/oxidative phosphorylation/redox-mitochondrial functions, respectively. Cross-comparison of in vivo and in vitro responses indicated the existence of tightly coregulated gene groups that may correspond to gene pathways.},
added-at = {2011-07-21T16:10:59.000+0200},
author = {Dimopoulos, G. and Christophides, G. K. and Meister, S. and Schultz, J. and White, K. P. and Barillas-Mury, C. and Kafatos, F. C.},
biburl = {https://www.bibsonomy.org/bibtex/2cd3e6156a665e9584f4f14401285fa2c/jschultz},
interhash = {4e567c858078fcfb547267159d85b12a},
intrahash = {cd3e6156a665e9584f4f14401285fa2c},
journal = {Proc. Natl. Acad. Sci. U.S.A.},
keywords = {imported},
month = Jun,
pages = {8814--8819},
timestamp = {2011-07-21T16:11:01.000+0200},
title = {{{G}enome expression analysis of {A}nopheles gambiae: responses to injury, bacterial challenge, and malaria infection}},
volume = 99,
year = 2002
}