%0 Journal Article %1 Moran:2014:Epigenetics:24732293 %A Moran, S %A Vizoso, M %A Martinez-Cardús, A %A Gomez, A %A Matías-Guiu, X %A Chiavenna, S M %A Fernandez, A G %A Esteller, M %D 2014 %J Epigenetics %K FFPE fulltext microarray %N 6 %P 829-833 %R 10.4161/epi.28790 %T Validation of DNA methylation profiling in formalin-fixed paraffin-embedded samples using the Infinium HumanMethylation450 Microarray %U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4065180/ %V 9 %X A formalin-fixed paraffin-embedded (FFPE) sample usually yields highly degraded DNA, which limits the use of techniques requiring high-quality DNA, such as Infinium Methylation microarrays. To overcome this restriction, we have applied an FFPE restoration procedure consisting of DNA repair and ligation processes in a set of paired fresh-frozen (FF) and FFPE samples. We validated the FFPE results in comparison with matched FF samples, enabling us to use FFPE samples on the Infinium HumanMethylation450 Methylation array.

@article{Moran:2014:Epigenetics:24732293, abstract = {A formalin-fixed paraffin-embedded (FFPE) sample usually yields highly degraded DNA, which limits the use of techniques requiring high-quality DNA, such as Infinium Methylation microarrays. To overcome this restriction, we have applied an FFPE restoration procedure consisting of DNA repair and ligation processes in a set of paired fresh-frozen (FF) and FFPE samples. We validated the FFPE results in comparison with matched FF samples, enabling us to use FFPE samples on the Infinium HumanMethylation450 Methylation array.}, added-at = {2017-06-06T19:21:02.000+0200}, author = {Moran, S and Vizoso, M and Martinez-Card{\'u}s, A and Gomez, A and Mat{\'i}as-Guiu, X and Chiavenna, S M and Fernandez, A G and Esteller, M}, biburl = {https://www.bibsonomy.org/bibtex/2d6b3264178c9f31eab633d1464f6a422/marcsaric}, description = {Validation of DNA methylation profiling in formalin-fixed paraffin-embedded samples using the Infinium HumanMethylation450 Microarray}, doi = {10.4161/epi.28790}, interhash = {a5afecb8743d2a4ca588037642cd9dd3}, intrahash = {d6b3264178c9f31eab633d1464f6a422}, journal = {Epigenetics}, keywords = {FFPE fulltext microarray}, month = jun, number = 6, pages = {829-833}, pmid = {24732293}, timestamp = {2017-11-06T17:22:50.000+0100}, title = {Validation of DNA methylation profiling in formalin-fixed paraffin-embedded samples using the Infinium HumanMethylation450 Microarray}, url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4065180/}, volume = 9, year = 2014 }