%0 Journal Article %1 Deprimo.2003 %A Deprimo, S. E. %A Wong, L. M. %A Khatry, D. B. %A Nicholas, S. L. %A Manning, W. C. %A Smolich, B. D. %A O'Farrell, A. M. %A Cherrington, J. M. %D 2003 %J BMC.Cancer %K & 9 Aged Analysis Angiogenesis Antigens Array Biological Biopsy CD CD24 Chain Chemistry Clinical Colorectal Expression Female Gene Glycoproteins Human Humans III Indoles Inhibitors Kinase Kinases Lactoferrin Leukocytes Male Markers Matrix Membrane Metalloproteinase Metastasis Middle Mononuclear Neoplasm Neoplasms Neoplastic Oligonucleotide Phase Polymerase Predictive Profiling Protein-Tyrosine Pyrroles Reaction Regulation Research Reverse Sequence Tests Topic Transcriptase Trials Tumor Tyrosine Value analysis antagonists as blood cells drug genetics identify inhibitors metabolism methods of protein response therapeutic therapy use %P 3 %T Expression profiling of blood samples from an SU5416 Phase III metastatic colorectal cancer clinical trial: a novel strategy for biomarker identification %U PM:12657164 %V 3 %X BACKGROUND: Microarray-based gene expression profiling is a powerful approach for the identification of molecular biomarkers of disease, particularly in human cancers. Utility of this approach to measure responses to therapy is less well established, in part due to challenges in obtaining serial biopsies. Identification of suitable surrogate tissues will help minimize limitations imposed by those challenges. This study describes an approach used to identify gene expression changes that might serve as surrogate biomarkers of drug activity. METHODS: Expression profiling using microarrays was applied to peripheral blood mononuclear cell (PBMC) samples obtained from patients with advanced colorectal cancer participating in a Phase III clinical trial. The PBMC samples were harvested pre-treatment and at the end of the first 6-week cycle from patients receiving standard of care chemotherapy or standard of care plus SU5416, a vascular endothelial growth factor (VEGF) receptor tyrosine kinase

@article{Deprimo.2003, abstract = {BACKGROUND: Microarray-based gene expression profiling is a powerful approach for the identification of molecular biomarkers of disease, particularly in human cancers. Utility of this approach to measure responses to therapy is less well established, in part due to challenges in obtaining serial biopsies. Identification of suitable surrogate tissues will help minimize limitations imposed by those challenges. This study describes an approach used to identify gene expression changes that might serve as surrogate biomarkers of drug activity. METHODS: Expression profiling using microarrays was applied to peripheral blood mononuclear cell (PBMC) samples obtained from patients with advanced colorectal cancer participating in a Phase III clinical trial. The PBMC samples were harvested pre-treatment and at the end of the first 6-week cycle from patients receiving standard of care chemotherapy or standard of care plus SU5416, a vascular endothelial growth factor (VEGF) receptor tyrosine kinase }, added-at = {2010-02-05T11:28:39.000+0100}, author = {Deprimo, S. E. and Wong, L. M. and Khatry, D. B. and Nicholas, S. L. and Manning, W. C. and Smolich, B. D. and O'Farrell, A. M. and Cherrington, J. M.}, biburl = {https://www.bibsonomy.org/bibtex/2d7eb1e499a929ad11a38f6275522e3c3/kanefendt}, interhash = {b23a3bc6b9b242ec9742ca38882aa95d}, intrahash = {d7eb1e499a929ad11a38f6275522e3c3}, journal = {BMC.Cancer}, keywords = {& 9 Aged Analysis Angiogenesis Antigens Array Biological Biopsy CD CD24 Chain Chemistry Clinical Colorectal Expression Female Gene Glycoproteins Human Humans III Indoles Inhibitors Kinase Kinases Lactoferrin Leukocytes Male Markers Matrix Membrane Metalloproteinase Metastasis Middle Mononuclear Neoplasm Neoplasms Neoplastic Oligonucleotide Phase Polymerase Predictive Profiling Protein-Tyrosine Pyrroles Reaction Regulation Research Reverse Sequence Tests Topic Transcriptase Trials Tumor Tyrosine Value analysis antagonists as blood cells drug genetics identify inhibitors metabolism methods of protein response therapeutic therapy use}, pages = 3, timestamp = {2010-02-05T11:28:40.000+0100}, title = {Expression profiling of blood samples from an SU5416 Phase III metastatic colorectal cancer clinical trial: a novel strategy for biomarker identification}, url = {PM:12657164}, volume = 3, year = 2003 }