Caulobacter crescentus is a model organism for the integrated circuitry that runs a bacterial cell cycle. Full discovery of its essential genome, including non-coding, regulatory and coding elements, is a prerequisite for understanding the complete regulatory network of a bacterial cell. Using hyper-saturated transposon mutagenesis coupled with high-throughput sequencing, we determined the essential Caulobacter genome at 8 bp resolution, including 1012 essential genome features: 480 ORFs, 402 regulatory sequences and 130 non-coding elements, including 90 intergenic segments of unknown function. The essential transcriptional circuitry for growth on rich media includes 10 transcription factors, 2 RNA polymerase sigma factors and 1 anti-sigma factor. We identified all essential promoter elements for the cell cycle-regulated genes. The essential elements are preferentially positioned near the origin and terminus of the chromosome. The high-resolution strategy used here is applicable to high-throughput, full genome essentiality studies and large-scale genetic perturbation experiments in a broad class of bacterial species.
%0 Journal Article
%1 Christen2011Essential
%A Christen, Beat
%A Abeliuk, Eduardo
%A Collier, John M.
%A Kalogeraki, Virginia S.
%A Passarelli, Ben
%A Coller, John A.
%A Fero, Michael J.
%A McAdams, Harley H.
%A Shapiro, Lucy
%D 2011
%I Nature Publishing Group
%J Molecular systems biology
%K minimal-genome synthetic-biology
%N 1
%R 10.1038/msb.2011.58
%T The essential genome of a bacterium.
%U http://dx.doi.org/10.1038/msb.2011.58
%V 7
%X Caulobacter crescentus is a model organism for the integrated circuitry that runs a bacterial cell cycle. Full discovery of its essential genome, including non-coding, regulatory and coding elements, is a prerequisite for understanding the complete regulatory network of a bacterial cell. Using hyper-saturated transposon mutagenesis coupled with high-throughput sequencing, we determined the essential Caulobacter genome at 8 bp resolution, including 1012 essential genome features: 480 ORFs, 402 regulatory sequences and 130 non-coding elements, including 90 intergenic segments of unknown function. The essential transcriptional circuitry for growth on rich media includes 10 transcription factors, 2 RNA polymerase sigma factors and 1 anti-sigma factor. We identified all essential promoter elements for the cell cycle-regulated genes. The essential elements are preferentially positioned near the origin and terminus of the chromosome. The high-resolution strategy used here is applicable to high-throughput, full genome essentiality studies and large-scale genetic perturbation experiments in a broad class of bacterial species.
@article{Christen2011Essential,
abstract = {
Caulobacter crescentus is a model organism for the integrated circuitry that runs a bacterial cell cycle. Full discovery of its essential genome, including non-coding, regulatory and coding elements, is a prerequisite for understanding the complete regulatory network of a bacterial cell. Using hyper-saturated transposon mutagenesis coupled with high-throughput sequencing, we determined the essential Caulobacter genome at 8 bp resolution, including 1012 essential genome features: 480 {ORFs}, 402 regulatory sequences and 130 non-coding elements, including 90 intergenic segments of unknown function. The essential transcriptional circuitry for growth on rich media includes 10 transcription factors, 2 {RNA} polymerase sigma factors and 1 anti-sigma factor. We identified all essential promoter elements for the cell cycle-regulated genes. The essential elements are preferentially positioned near the origin and terminus of the chromosome. The high-resolution strategy used here is applicable to high-throughput, full genome essentiality studies and large-scale genetic perturbation experiments in a broad class of bacterial species.
},
added-at = {2018-12-02T16:09:07.000+0100},
author = {Christen, Beat and Abeliuk, Eduardo and Collier, John M. and Kalogeraki, Virginia S. and Passarelli, Ben and Coller, John A. and Fero, Michael J. and McAdams, Harley H. and Shapiro, Lucy},
biburl = {https://www.bibsonomy.org/bibtex/2e49dfef46638d4c88c78910441f24467/karthikraman},
citeulike-article-id = {9729226},
citeulike-linkout-0 = {http://dx.doi.org/10.1038/msb.2011.58},
citeulike-linkout-1 = {http://dx.doi.org/10.1038/msb201158},
citeulike-linkout-2 = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3202797/},
citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/21878915},
citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=21878915},
day = 30,
doi = {10.1038/msb.2011.58},
interhash = {1b61287d9af45a17e0f54a3c63974d74},
intrahash = {e49dfef46638d4c88c78910441f24467},
issn = {1744-4292},
journal = {Molecular systems biology},
keywords = {minimal-genome synthetic-biology},
month = aug,
number = 1,
pmcid = {PMC3202797},
pmid = {21878915},
posted-at = {2011-12-12 08:50:34},
priority = {2},
publisher = {Nature Publishing Group},
timestamp = {2018-12-02T16:09:07.000+0100},
title = {The essential genome of a bacterium.},
url = {http://dx.doi.org/10.1038/msb.2011.58},
volume = 7,
year = 2011
}